Key features and details
- Rabbit polyclonal to Pericentrin
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pericentrin antibody
See all Pericentrin primary antibodies
DescriptionRabbit polyclonal to Pericentrin
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human testis tissue. ICC/IF: A431 cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionIntegral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
Tissue specificityExpressed in all tissues tested, including placenta, liver, kidney and thymus.
Involvement in diseaseMicrocephalic osteodysplastic primordial dwarfism 2
DomainComposed of a coiled-coil central region flanked by non-helical N- and C-terminals.
Cellular localizationCytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
- Information by UniProt
- Centrosome Marker antibody
- Ken antibody
- Kendrin antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Pericentrin (green) using ab224101 (4 µg/ml) in ICC/IF.
Immunohistochemical analysis of human testis tissue labeling Pericentrin with ab224101 at 1:500 dilution.
Immunohistochemical analysis of human liver tissue labeling Pericentrin with ab224101 at 1:500 dilution. Negative control showing no cytoplasmic positivity in hepatocytes.
Immunohistochemical analysis of human placenta tissue labeling Pericentrin with ab224101 at 1:500 dilution.
Immunohistochemical analysis of human cerebral cortex tissue labeling Pericentrin with ab224101 at 1:500 dilution.
ab224101 has not yet been referenced specifically in any publications.