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    pericentrin-antibody-centrosome-marker-ab4448.pdf

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Cell Biology Cell Cycle Cell Division Spindle
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Anti-Pericentrin antibody - Centrosome Marker (ab4448)

  • Datasheet
  • SDS
Reviews (32)Q&A (5)References (360)

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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

Key features and details

  • Rabbit polyclonal to Pericentrin - Centrosome Marker
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Pericentrin antibody [EPR21987] (ab220784)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Pericentrin antibody - Centrosome Marker
    See all Pericentrin primary antibodies
  • Description

    Rabbit polyclonal to Pericentrin - Centrosome Marker
  • Host species

    Rabbit
  • Specificity

    This antibody should recognise both Pericentrin and Kendrin (also known as Pericentrin-2).
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Fusion protein corresponding to Mouse Pericentrin aa 100-600.

  • Positive control

    • ICC: Human coronary artery endothelial, MCF7, HeLa and NIH/3T3 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Centrosome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
Human
All applications
Rat
Rabbit
African green monkey
Application Abreviews Notes
ICC/IF (27)
Use a concentration of 0.1 - 0.5 µg/ml.
Notes
ICC/IF
Use a concentration of 0.1 - 0.5 µg/ml.

Target

  • Function

    Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
  • Tissue specificity

    Expressed in all tissues tested, including placenta, liver, kidney and thymus.
  • Involvement in disease

    Microcephalic osteodysplastic primordial dwarfism 2
  • Domain

    Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
  • Cellular localization

    Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
  • Target information above from: UniProt accession O95613 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5116 Human
    • Entrez Gene: 18541 Mouse
    • Omim: 605925 Human
    • SwissProt: O95613 Human
    • SwissProt: P48725 Mouse
    • Unigene: 474069 Human
    • Unigene: 251794 Mouse
    • Alternative names

      • Centrosome Marker antibody
      • Ken antibody
      • Kendrin antibody
      • KIAA0402 antibody
      • MOPD2 antibody
      • PCN antibody
      • PCNT 2 antibody
      • PCNT antibody
      • PCNT B antibody
      • PCNT_HUMAN antibody
      • PCNT1 antibody
      • PCNT2 antibody
      • PCNTB antibody
      • PCTN2 antibody
      • Pericentrin 1 antibody
      • Pericentrin 2 antibody
      • Pericentrin 380 antibody
      • Pericentrin antibody
      • Pericentrin B antibody
      • Pericentrin-B antibody
      • SCKL4 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)Image courtesy of an anonymous abreview

      Human coronary artery endothelial cells (HCAEC) were labeled for Pericentrin using ab4448 at 1/100 dilution in ICC/IF. Primary incubation was for 1 hour at room temperature (green). The seciondary antibody was a Goat anti-rabbit IgG AlexaFluor® conjugate.

      Fixation: Formaldehyde. Permeabilisation: 0.1% Trition X-100 in HBSS.

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)Ruppenthal et al PLoS One. 2018 Jan 25;13(1):e0191734. doi: 10.1371/journal.pone.0191734. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      A representative panel of indirect immunofluorescence microscopic images shows normal (regular, n ≤ 2) and aberrant centrosome numbers (n > 2) in interphase cells.

      Centrosomes were stained using anti-pericentrin antibody ab4448 (magenta), nuclear DNA is shown in blue (DAPI).

      Statistical methods: Kruskal-Wallis test. Mann-Whitney U tests followed by Bonferroni-Holm p-value correction were made as post-hoc tests in order to compare the MDS and sAML patients with the control group.

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)This image is courtesy of Gordon Chan, University of Alberta

      IF staining of pericentrin in MCF7 (Human breast adenocarcinoma cell line) cells.

      The top panel is an interphase cell showing centrosome staining. 

      The bottom panel shows a mitotic cell with spindle pole staining. 

      ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).

      Top panel - 630X magnification; Bottom panel -1000X magnification.

      The secondary antibody was Alexa-Fluor®488 anti-rabbit.

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)This image is courtesy of Roberto Giambruno, Marilena Ciciarello and Patrizia Lavia

      NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature.

      The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

      ICC/IF image of ab4448 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

      The cells were fixed in methanol (5 minutes) and incubated with the antibody (ab4448, 1 µg/ml) for 1 hour at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

      ab4448 stained NIH/3T3 (Mouse embryo fibroblast cell line) cells.

      The cells were fixed in 100% methanol for 5 minutes at -20°C and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448 at 5 µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature.

      DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1 hour at room temperature.

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

      ICC/IF image of ab4448 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

      The cells were fixed in 100% methanol (5 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour.

      DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Recommended ICC/IF protocol with ab4448

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (360)

    Publishing research using ab4448? Please let us know so that we can cite the reference in this datasheet.

    ab4448 has been referenced in 360 publications.

    • Huhn O  et al. Distinctive phenotypes and functions of innate lymphoid cells in human decidua during early pregnancy. Nat Commun 11:381 (2020). PubMed: 31959757
    • Denu RA  et al. Centrosome Amplification in Cancer Disrupts Autophagy and Sensitizes to Autophagy Inhibition. Mol Cancer Res 18:33-45 (2020). PubMed: 31604847
    • Naso FD  et al. Excess TPX2 Interferes with Microtubule Disassembly and Nuclei Reformation at Mitotic Exit. Cells 9:N/A (2020). PubMed: 32041138
    • Mikulenkova E  et al. NANOG/NANOGP8 Localizes at the Centrosome and is Spatiotemporally Associated with Centriole Maturation. Cells 9:N/A (2020). PubMed: 32168958
    • Wu D & Dean J EXOSC10 sculpts the transcriptome during the growth-to-maturation transition in mouse oocytes. Nucleic Acids Res 48:5349-5365 (2020). PubMed: 32313933
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 37 Abreviews or Q&A

    Western blot abreview for Anti-Pericentrin antibody - Centrosome Marker

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Dopaminergic Neurons, HEK293T and HeLa)
    Gel Running Conditions
    Reduced Denaturing (NuPage 4-12% MES buffer)
    Loading amount
    30 µg
    Specification
    Dopaminergic Neurons, HEK293T and HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 20 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human coronary artery endothelial cells HCAEC)
    Permeabilization
    Yes - 0.1% Trition X-100 in HBSS
    Specification
    Human coronary artery endothelial cells HCAEC
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 19 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HAP1)
    Permeabilization
    Yes - 0.5% Triton 5 minutes
    Specification
    HAP1
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 14 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (colon carcinoma cells)
    Permeabilization
    Yes - Triton
    Specification
    colon carcinoma cells
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 11 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (BS-C-1 cells)
    Permeabilization
    Yes - 0.2% Triton X
    Specification
    BS-C-1 cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. J. Y. Seo

    Verified customer

    Submitted Apr 09 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (brain section)
    Specification
    brain section
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 18 2017

    Western blot abreview for Anti-Pericentrin antibody - Centrosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Gel Running Conditions
    Reduced Denaturing (4-12 Bis tris)
    Loading amount
    5e+006 cells
    Specification
    293T
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 18 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HT1080)
    Permeabilization
    Yes - 0.5% Triton X100
    Specification
    HT1080
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 18 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody

    Poor
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse prostate and mammary epithelial)
    Specification
    mouse prostate and mammary epithelial
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
    Fixative
    try both PFA and methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 13 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Human Cell (MCF10A Breast Cancer Cell Line)
    Specification
    MCF10A Breast Cancer Cell Line
    Permeabilization
    No
    Fixative
    1% Gluteraldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 13 2014

    1-10 of 37 Abreviews or Q&A

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