Recombinant
RabMAb

Recombinant Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

Overview

  • Product name

    Anti-Pericentrin antibody [EPR21987] - BSA and Azide free
    See all Pericentrin primary antibodies
  • Description

    Rabbit monoclonal [EPR21987] to Pericentrin - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    IHC is recommended for human only.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Pericentrin aa 250-600. The exact sequence is proprietary.
    Database link: O95613

  • Positive control

    • IHC-P: Human breast tissue.
  • General notes

    Ab237034 is the carrier-free version of ab220784. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab237034 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab237034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
  • Tissue specificity

    Expressed in all tissues tested, including placenta, liver, kidney and thymus.
  • Involvement in disease

    Microcephalic osteodysplastic primordial dwarfism 2
  • Domain

    Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
  • Cellular localization

    Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Centrosome Marker antibody
    • Ken antibody
    • Kendrin antibody
    • KIAA0402 antibody
    • MOPD2 antibody
    • PCN antibody
    • PCNT 2 antibody
    • PCNT antibody
    • PCNT B antibody
    • PCNT_HUMAN antibody
    • PCNT1 antibody
    • PCNT2 antibody
    • PCNTB antibody
    • PCTN2 antibody
    • Pericentrin 1 antibody
    • Pericentrin 2 antibody
    • Pericentrin 380 antibody
    • Pericentrin antibody
    • Pericentrin B antibody
    • Pericentrin-B antibody
    • SCKL4 antibody
    see all

Images

  • Immunofluorescent analysis of 100% Methanol-fixed NIH/3T3 (mouse embyro fibroblast cell line) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in NIH/3T3 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

  • Pericentrin was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate with ab220784 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220784 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 μg (input).
    Lane 2: ab220784 IP in NIH/3T3 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220784 in NIH/3T3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 30 seconds

    The 220 kDa band represent an isoform of Pericentrin (PMID: 23060948). We use high sensitive ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

  • Pericentrin was immunoprecipitated from 0.35 mg HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab220784 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220784 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HepG2 whole cell lysate 10 μg (input).
    Lane 2: ab220784 IP in HepG2 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220784 in HepG2 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 30 seconds

    The 220 kDa band represent an isoform of Pericentrin (PMID: 23060948). We used a high sensitivity ECL substrate to develop this blot.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Pericentrin with ab220784 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

  • Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human thyroid carcinoma (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human pancreas (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 100% Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in HeLa cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

  • Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human mammary epithelial cells (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab237034 has not yet been referenced specifically in any publications.

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