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Recombinant

Recombinant Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)

  • Datasheet
  • SDS
Reviews (11)Q&A (1)References (62)

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Immunocytochemistry - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
  • Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [mAbcam 28144] to Pericentrin - Centrosome Marker
  • Suitable for: ICC
  • Reacts with: Human

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Overview

  • Product name

    Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker
    See all Pericentrin primary antibodies
  • Description

    Mouse monoclonal [mAbcam 28144] to Pericentrin - Centrosome Marker
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC
    Human
    See all applications and species data
  • Immunogen

    Fusion protein corresponding to Mouse Pericentrin aa 100-600.

  • Positive control

    • ICC: HeLa cells.
  • General notes

    This product has switched from a hybridoma to recombinant production method on 9th September 2020.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 28144
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Centrosome

Associated products

  • Alternative Versions

    • Anti-Pericentrin antibody [mAbcam 28144] - BSA and Azide free (ab256363)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
All applications
Chinese hamster
Application Abreviews Notes
ICC (1)
Use a concentration of 1 µg/ml.
Notes
ICC
Use a concentration of 1 µg/ml.

Target

  • Function

    Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
  • Tissue specificity

    Expressed in all tissues tested, including placenta, liver, kidney and thymus.
  • Involvement in disease

    Microcephalic osteodysplastic primordial dwarfism 2
  • Domain

    Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
  • Cellular localization

    Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
  • Target information above from: UniProt accession O95613 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5116 Human
    • Omim: 605925 Human
    • SwissProt: O95613 Human
    • Unigene: 474069 Human
    • Alternative names

      • Centrosome Marker antibody
      • Ken antibody
      • Kendrin antibody
      • KIAA0402 antibody
      • MOPD2 antibody
      • PCN antibody
      • PCNT 2 antibody
      • PCNT antibody
      • PCNT B antibody
      • PCNT_HUMAN antibody
      • PCNT1 antibody
      • PCNT2 antibody
      • PCNTB antibody
      • PCTN2 antibody
      • Pericentrin 1 antibody
      • Pericentrin 2 antibody
      • Pericentrin 380 antibody
      • Pericentrin antibody
      • Pericentrin B antibody
      • Pericentrin-B antibody
      • SCKL4 antibody
      see all

    Images

    • Immunocytochemistry - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
      Immunocytochemistry - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)

      Immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab28144 at 1/50 dilution at 4°C overnight, followed by AlexaFluor® 488-conjugated Goat anti-Rabbit secondary
      antibody (ab150077) at 1/1000 dilution at RT for 30min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] (ab195889) was used as a counterstain at 1/200 dilution at RT for 30min. Nucleus were visualized using DAPI. The cells were 4% PFA fixed and permeabilized with 0.1% Triton X-100.

      Confocal image showing centrosome staining in HeLa cells.

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)Image from Sikkema, William K. A. et al. PLoS ONE 9.6 (2014): e98838. PMC. Web. 24 Feb. 2017. doi: 10.1371/journal.pone.0098838. Fig 4F. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunocytochemistry/ Immunofluorescence analysis of Y79 cells labeling Pericentrin with ab28144 (red). Cells were fixed for 10 minutes at room temperature in 4% paraformaldehyde. Cells were then permeabilized using 0.2% Triton/TBS and blocked with 5% NGS in 0.1% BSA/TBS-Tween. The cells were then incubated overnight at 4°C with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) at 1/1000 diluted in 0.1% BSA/TBS-Tween containing 1% NGS. Cells were then stained for Hoechst (blue) and alpha-tubulin (green).

      This image was generated from the hybridoma version.

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)This image is courtesy of an anonymous abreview.

      Immunocytochemistry/ Immunofluorescence analysis of A549 lung cells labeling Pericentrin with ab28144 at 1/100 dilution. Cells were fixed with formaldehyde and permeabilized with Trixton X-100. The cells were blocked with 10% serum for 30 minutes at 20°C, followed by incubation with ab28144 in PBS 10% FCS for 1 hour at 20°C. A polyclonal goat anti-mouse Alexa Fluor® 594 secondary antibody was used at 1/300 dilution.

       This image was generated from the hybridoma version.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)This image is courtesy of an Abreview submitted by Dr Kirk McManus, Univ. of Manitoba/Cancer Care MICB.

      Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Pericentrin with ab28144 at 1/200 dilution. Cells were fixed with methanol, followed by incubation with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) in PBS for 1 hour at 22°C. A polyclonal goat anti-mouse Cy3® secondary antibody was used at 1/200 dilution. Nuclear counterstained with DAPI.

      This image was generated from the hybridoma version.

      See Abreview

    • Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
      Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (62)

    Publishing research using ab28144? Please let us know so that we can cite the reference in this datasheet.

    ab28144 has been referenced in 62 publications.

    • Kim YH  et al. A MST1-FOXO1 cascade establishes endothelial tip cell polarity and facilitates sprouting angiogenesis. Nat Commun 10:838 (2019). PubMed: 30783090
    • Watanabe K  et al. The Cep57-pericentrin module organizes PCM expansion and centriole engagement. Nat Commun 10:931 (2019). PubMed: 30804344
    • Nardi F  et al. DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130. Sci Rep 9:3035 (2019). PubMed: 30816200
    • Full F  et al. Centrosomal protein TRIM43 restricts herpesvirus infection by regulating nuclear lamina integrity. Nat Microbiol 4:164-176 (2019). PubMed: 30420784
    • Molinari E  et al. Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts. Sci Rep 9:10828 (2019). PubMed: 31346239
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Primary Fibroblasts)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    Human Primary Fibroblasts
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 27 2020

    Immunoprecipitation abreview for Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - other (T98G cells)
    Total protein in input
    800 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    T98G cells
    Read More

    Dr. Julia Mills

    Verified customer

    Submitted Jul 02 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa Cells)
    Permeabilization
    Yes - 0.3% Triton
    Specification
    HeLa Cells
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Federico Dona

    Verified customer

    Submitted Nov 19 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 09 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HT1080)
    Permeabilization
    Yes - 0.2% Triton
    Specification
    HT1080
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 25 2017

    Immunocytochemistry abreview for Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HeLa)
    Permeabilization
    Yes - 02% triton
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 25 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody [mAbcam 28144]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Human Cell (HCT116)
    Specification
    HCT116
    Permeabilization
    Yes - when Paraformaldehyde
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 17 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker

    Poor
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Young adult mouse colonic epithelium (YAMC) cells)
    Permeabilization
    No
    Specification
    Young adult mouse colonic epithelium (YAMC) cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 25 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody [mAbcam 28144]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Methanol
    Permeabilization
    No
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Oct 14 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody [mAbcam 28144]

    Poor
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Cor1 cells)
    Specification
    Cor1 cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - TBS/BSA/azide/0.1% Triton
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 26 2010

    1-10 of 12 Abreviews or Q&A

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