Anti-Perilipin-1 antibody (ab126639)
Key features and details
- Rabbit polyclonal to Perilipin-1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Perilipin-1 antibody
See all Perilipin-1 primary antibodies -
Description
Rabbit polyclonal to Perilipin-1 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB MouseRat -
Immunogen
Synthetic peptide within Human Perilipin-1 aa 100-200 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O60240 -
Positive control
- WB: Mouse Brown Adipose (BAT) P7 and P0 tissue lysates. IHC-P: Human breast adipose tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IHC-P |
Human
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WB |
Mouse
Rat
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All applications |
Horse
Cow
Dog
Pig
Chimpanzee
Macaque monkey
Gorilla
Chinese hamster
Orangutan
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Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. -
Tissue specificity
Adipocytes. -
Sequence similarities
Belongs to the perilipin family. -
Post-translational
modificationsMajor cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. -
Cellular localization
Lipid droplet. Lipid droplet surface-associated. - Information by UniProt
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Database links
- Entrez Gene: 520598 Cow
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Entrez Gene: 25629 Rat
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- SwissProt: P43884 Rat
see all -
Alternative names
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
Images
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All lanes : Anti-Perilipin-1 antibody (ab126639) at 1 µg/ml
Lane 1 : P0 Mouse Brown Adipose Tissue Mouse Tissue Lysate
Lane 2 : P7 Rat Brown Adipose Tissue Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThe predicted molecular weight of Perilipin-1 is 56 kDa (SwissProt), however we expect to observe a banding pattern around 62 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab126639 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab126639)
IHC image of Perilipin-1 staining in human breast adipose formalin fixed paraffin embedded tissue, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126639, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (1)
ab126639 has been referenced in 1 publication.
- Faglin P et al. Rationale for the design of 3D-printable bioresorbable tissue-engineering chambers to promote the growth of adipose tissue. Sci Rep 10:11779 (2020). PubMed: 32678237