Anti-Perilipin-1 antibody (ab3526)
Key features and details
- Rabbit polyclonal to Perilipin-1
- Suitable for: Flow Cyt, IHC-P, WB, ICC, IHC-FoFr
- Reacts with: Mouse, Rat, Cow, Dog, Human, Rhesus monkey
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Perilipin-1 antibody
See all Perilipin-1 primary antibodies -
Description
Rabbit polyclonal to Perilipin-1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WB, ICC, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Dog, Human, Rhesus monkey -
Immunogen
Synthetic peptide corresponding to Rat Perilipin-1 aa 500-600.
(Peptide available asab5009) -
Positive control
- ICC: mouse 3T3-L1 cells; WB: mouse 3T3-L1 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.01% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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IHC-P | (6) |
Use at an assay dependent concentration.
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WB | (2) |
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009).
By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
ICC |
Use a concentration of 2 µg/ml.
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|
IHC-FoFr |
Use at an assay dependent concentration. PubMed: 20049087
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009). By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
ICC
Use a concentration of 2 µg/ml. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20049087 |
Target
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Function
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. -
Tissue specificity
Adipocytes. -
Sequence similarities
Belongs to the perilipin family. -
Post-translational
modificationsMajor cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. -
Cellular localization
Lipid droplet. Lipid droplet surface-associated. - Information by UniProt
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Database links
- Entrez Gene: 520598 Cow
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Entrez Gene: 25629 Rat
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- SwissProt: P43884 Rat
see all -
Alternative names
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
Images
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Immunocytochemistry analysis of 3T3-L1 cells (Mouse embryonic fibroblast cell line), 4% paraformaldehyde fixed (10 minutes) permeabilized with 0.1% Triton™ X-100 (10 minutes), and blocked with 1% BSA for (1 hour).
Perilipin A was labeled with ab3526 at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature with secondary antibody: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel A: green).
Panel B: Nuclei were stained with DAPI (blue); Panel C: (red) F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin 1:300. Panel D: merged image showing cytosolic localization; Panel E: untreated; Panel F: control cells with no primary antibody.
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Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (111)
ab3526 has been referenced in 111 publications.
- Karadeniz F et al. Dracunculin Inhibits Adipogenesis in Human Bone Marrow-Derived Mesenchymal Stromal Cells by Activating AMPK and Wnt/β-Catenin Signaling. Int J Mol Sci 23:N/A (2022). PubMed: 35054838
- Zhao Y et al. Expression of Recombinant Rat Secretable FNDC5 in Pichia Pastoris and Detection of Its Biological Activity. Front Endocrinol (Lausanne) 13:852015 (2022). PubMed: 35321332
- Gautheron J et al. Loss of thymidine phosphorylase activity disrupts adipocyte differentiation and induces insulin-resistant lipoatrophic diabetes. BMC Med 20:95 (2022). PubMed: 35341481
- Niu X et al. Preoperative Short-term High-Carbohydrate Diet Provides More High-Quality Transplantable Fat and Improves the Outcome of Fat Grafts in Mice. Aesthet Surg J 42:NP531-NP545 (2022). PubMed: 35460566
- Tang W et al. Investigating the Adipogenic Effects of Different Tissue-Derived Decellularized Matrices. Front Bioeng Biotechnol 10:872897 (2022). PubMed: 35497363