Overview

  • Product name
  • Description
    Rabbit polyclonal to Perilipin-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IHC-FoFr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Rat Perilipin-1 aa 502-517.
    Sequence:

    EPILGRTQYSQLRKKS


    (Peptide available as ab5009)

Properties

Applications

Our Abpromise guarantee covers the use of ab3526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009).

By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract.

IHC-FoFr Use at an assay dependent concentration. PubMed: 20049087
ICC/IF Use at an assay dependent concentration. PubMed: 20335253
Flow Cyt Use 3-5µg for 106 cells.

Target

  • Function
    Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
  • Tissue specificity
    Adipocytes.
  • Sequence similarities
    Belongs to the perilipin family.
  • Post-translational
    modifications
    Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
  • Cellular localization
    Lipid droplet. Lipid droplet surface-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lipid droplet associated protein antibody
    • Lipid droplet-associated protein antibody
    • PERI antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin-1 antibody
    • PerilipinA antibody
    • PLIN antibody
    • PLIN1 antibody
    • PLIN1_HUMAN antibody
    see all

Images

  • ab3526 staining Perilipin-1 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in an EDTA buffer. Samples were incubated with primary antibody (1/200 in PBS) for 12 hours at 4°C. A HRP-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.

  • ab3526 staining Perilipin-1 in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml in 0.1% BSA) for 3 hours at room temperature and labeled with Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (Green, panel A). Nuclei stained with DAPI (Blue, panel B), F-actin stained with Alexa Fluor® 555 rhodamine phalloidin (Red, panel C), merged images showing cytosolic localization (panel D). 

  • ab3526 staining Perilipin-1 in 3T3-L1 cells by Flow Cytometry. Cells were fixed with 70% ethanol, permeablized with 0.25% Triton X-100 and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/million cells) for 2 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit was used as the secondary antibody (1:400), red histogram. Rabbit isotype control (pink histogram), unstained control (purple histogram) and no primary antibody control (green histogram).

  • ab3526 (4µg/ml) staining Perilipin-1 in Human breast adipose using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of adipocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Immunofluorescence analysis of Human preadipocytes, staining Perilipin-1 with ab3526. An Alexa Fluor® 568-conjugated anti-rabbit IgG was used as the secondary antibody.

  • Immunohistochemical analysis of dog mammary adipose tissue, staining Perilipin-1 with ab3526.

    Tissue was fixed with formaldehyde and blocked with 1% blocking solution for 15 minutes at room temperature; antigen retrieval was by heat mediation in Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/200 in BSA in TBS) for 30 minutes. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • ab3526 staining Perilipin-1 in rat myofibers by Immunohistochemistry (PFA fixed).Freshly isolated single myofibers were collected in 0.5 ml DMEM, 5% HS in an Eppendorf tube. Fibers and satellite cells were fixed with 4% paraformaldehyde in phosphate buffered saline, rinsed in PBS and permeabilized with Triton X-100, 0.5% in PBS. After washing, fibers and/or cells were incubated with ab3526 at a 1/50 dilution overnight at 4°C or 1 hour at 37°C. Non specific interactions were blocked with 20% goat serum. They were then washed and incubated with labeled secondary antibodies for one hour at room temperature. Satellite cells were then mounted with fluorescent mounting medium plus DAPI 100 ng/ml. Fibers were collected and moved onto a polilysine microscope slide, and then mounted.

References

This product has been referenced in:
  • Tosic M  et al. Lsd1 regulates skeletal muscle regeneration and directs the fate of satellite cells. Nat Commun 9:366 (2018). Read more (PubMed: 29371665) »
  • Kosacka J  et al. Up-regulated autophagy: as a protective factor in adipose tissue of WOKW rats with metabolic syndrome. Diabetol Metab Syndr 10:13 (2018). Read more (PubMed: 29507613) »
See all 40 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Immunohistochemistry (Resin sections)
Sample
Human Tissue sections (adipocytes)
Specification
adipocytes

Dr. M Malatesta

Verified customer

Submitted Dec 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (fat tissue)
Permeabilization
No
Specification
fat tissue
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 10 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 10 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (adipose)
Antigen retrieval step
Other
Permeabilization
Yes - 30 min 1x PBS 0.5% tritron buffer
Specification
adipose
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Mammary Adipose Tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9 EDTA
Permeabilization
No
Specification
Mammary Adipose Tissue
Blocking step
DAKO PB as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 30 2012

Answer

Thank you for your reply.

Ypu are correct. The expiration is 27 March 2013.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab3526 in Canine. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for Canine. The code is inactive until included in your submitted Abreview so be sure to add it to the Additional Comments section. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/AbTrial

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (3T3-L1)
Specification
3T3-L1
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 27 2006

1-10 of 11 Abreviews or Q&A

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