Anti-Perilipin-1 antibody (ab3526)
Key features and details
- Rabbit polyclonal to Perilipin-1
- Suitable for: IHC-P, WB, ICC, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Perilipin-1 antibody
See all Perilipin-1 primary antibodies -
Description
Rabbit polyclonal to Perilipin-1 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt MouseICC MouseIHC-P HumanWB Mouse -
Immunogen
Synthetic peptide corresponding to Rat Perilipin-1 aa 500-600.
(Peptide available asab5009)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
Flow Cyt |
Mouse
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ICC |
Mouse
|
IHC-P |
Human
|
WB |
Mouse
|
All applications |
Dog
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Application | Abreviews | Notes |
---|---|---|
IHC-P | (6) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
WB |
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009).
By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
|
ICC |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use 3-5µg for 106 cells.
|
Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009). By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
ICC
Use at an assay dependent concentration. |
Flow Cyt
Use 3-5µg for 106 cells. |
Target
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Function
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. -
Tissue specificity
Adipocytes. -
Sequence similarities
Belongs to the perilipin family. -
Post-translational
modificationsMajor cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. -
Cellular localization
Lipid droplet. Lipid droplet surface-associated. - Information by UniProt
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Database links
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- Unigene: 103253 Human
- Unigene: 254917 Mouse
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Alternative names
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)This image is courtesy of an anonymous Abreview
ab3526 staining Perilipin-1 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in an EDTA buffer. Samples were incubated with primary antibody (1/200 in PBS) for 12 hours at 4°C. A HRP-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
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Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.
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ab3526 staining Perilipin-1 in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml in 0.1% BSA) for 3 hours at room temperature and labeled with Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (Green, panel A). Nuclei stained with DAPI (Blue, panel B), F-actin stained with Alexa Fluor® 555 rhodamine phalloidin (Red, panel C), merged images showing cytosolic localization (panel D).
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ab3526 staining Perilipin-1 in 3T3-L1 cells by Flow Cytometry. Cells were fixed with 70% ethanol, permeablized with 0.25% Triton X-100 and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/million cells) for 2 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit was used as the secondary antibody (1:400), red histogram. Rabbit isotype control (pink histogram), unstained control (purple histogram) and no primary antibody control (green histogram).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)
ab3526 (4µg/ml) staining Perilipin-1 in Human breast adipose using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of adipocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)Image from Stenson BM et al., J Biol Chem. 2011 Jan 7;286(1):370-9. doi: 10.1074/jbc.M110.179499. Epub 2010 Oct 28.; Fig 3.; January 7, 2011, The Journal of Biological Chemistry, 286, 370-379.
Immunofluorescence analysis of Human preadipocytes, staining Perilipin-1 with ab3526. An Alexa Fluor® 568-conjugated anti-rabbit IgG was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)This image is courtesy of an anonymous Abreview
Immunohistochemical analysis of dog mammary adipose tissue, staining Perilipin-1 with ab3526.
Tissue was fixed with formaldehyde and blocked with 1% blocking solution for 15 minutes at room temperature; antigen retrieval was by heat mediation in Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/200 in BSA in TBS) for 30 minutes. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
Protocols
References (64)
ab3526 has been referenced in 64 publications.
- Yang Y et al. O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun 11:181 (2020). PubMed: 31924761
- Lu C et al. Long non-coding RNA ARAP1-AS1 accelerates cell proliferation and migration in breast cancer through miR-2110/HDAC2/PLIN1 axis. Biosci Rep 40:N/A (2020). PubMed: 32110804
- Rodriguez BL et al. A tissue engineering approach for repairing craniofacial volumetric muscle loss in a sheep following a 2, 4, and 6-month recovery. PLoS One 15:e0239152 (2020). PubMed: 32956427
- Xu Q et al. Adipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactation1. J Anim Sci 97:2837-2849 (2019). PubMed: 31267132
- Lynn EG et al. 4-Phenylbutyrate protects against atherosclerotic lesion growth by increasing the expression of HSP25 in macrophages and in the circulation of Apoe-/- mice. FASEB J 33:8406-8422 (2019). PubMed: 30964709