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    perilipin-1-antibody-ab3526.pdf

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Cardiovascular Lipids / Lipoproteins Adipose Related Lipid Droplet Protein
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Anti-Perilipin-1 antibody (ab3526)

  • Datasheet
  • SDS
Reviews (10)Q&A (3)References (64)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)
  • Western blot - Anti-Perilipin-1 antibody (ab3526)
  • Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)
  • Flow Cytometry - Anti-Perilipin-1 antibody (ab3526)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)
  • Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)

Key features and details

  • Rabbit polyclonal to Perilipin-1
  • Suitable for: IHC-P, WB, ICC, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Perilipin-1 antibody
    See all Perilipin-1 primary antibodies
  • Description

    Rabbit polyclonal to Perilipin-1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Rat Perilipin-1 aa 500-600.
    (Peptide available as ab5009)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Lipid Droplet Protein
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • Perilipin-1 peptide (ab5009)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Mouse
ICC
Mouse
IHC-P
Human
WB
Mouse
All applications
Dog
Application Abreviews Notes
IHC-P (6)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009).

By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract.

ICC
Use at an assay dependent concentration.
Flow Cyt
Use 3-5µg for 106 cells.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009).

By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract.

ICC
Use at an assay dependent concentration.
Flow Cyt
Use 3-5µg for 106 cells.

Target

  • Function

    Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
  • Tissue specificity

    Adipocytes.
  • Sequence similarities

    Belongs to the perilipin family.
  • Post-translational
    modifications

    Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
  • Cellular localization

    Lipid droplet. Lipid droplet surface-associated.
  • Target information above from: UniProt accession O60240 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5346 Human
    • Entrez Gene: 103968 Mouse
    • Omim: 170290 Human
    • SwissProt: O60240 Human
    • SwissProt: Q8CGN5 Mouse
    • Unigene: 103253 Human
    • Unigene: 254917 Mouse
    • Alternative names

      • Lipid droplet associated protein antibody
      • Lipid droplet-associated protein antibody
      • PERI antibody
      • Perilipin A antibody
      • Perilipin antibody
      • Perilipin-1 antibody
      • PerilipinA antibody
      • PLIN antibody
      • PLIN1 antibody
      • PLIN1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)This image is courtesy of an anonymous Abreview

      ab3526 staining Perilipin-1 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in an EDTA buffer. Samples were incubated with primary antibody (1/200 in PBS) for 12 hours at 4°C. A HRP-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Western blot - Anti-Perilipin-1 antibody (ab3526)
      Western blot - Anti-Perilipin-1 antibody (ab3526)

      Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.

    • Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)
      Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)

      ab3526 staining Perilipin-1 in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml in 0.1% BSA) for 3 hours at room temperature and labeled with Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (Green, panel A). Nuclei stained with DAPI (Blue, panel B), F-actin stained with Alexa Fluor® 555 rhodamine phalloidin (Red, panel C), merged images showing cytosolic localization (panel D). 

    • Flow Cytometry - Anti-Perilipin-1 antibody (ab3526)
      Flow Cytometry - Anti-Perilipin-1 antibody (ab3526)

      ab3526 staining Perilipin-1 in 3T3-L1 cells by Flow Cytometry. Cells were fixed with 70% ethanol, permeablized with 0.25% Triton X-100 and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/million cells) for 2 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit was used as the secondary antibody (1:400), red histogram. Rabbit isotype control (pink histogram), unstained control (purple histogram) and no primary antibody control (green histogram).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)

      ab3526 (4µg/ml) staining Perilipin-1 in Human breast adipose using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of adipocytes.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    • Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)
      Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)Image from Stenson BM et al., J Biol Chem. 2011 Jan 7;286(1):370-9. doi: 10.1074/jbc.M110.179499. Epub 2010 Oct 28.; Fig 3.; January 7, 2011, The Journal of Biological Chemistry, 286, 370-379.

      Immunofluorescence analysis of Human preadipocytes, staining Perilipin-1 with ab3526. An Alexa Fluor® 568-conjugated anti-rabbit IgG was used as the secondary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody (ab3526)This image is courtesy of an anonymous Abreview

      Immunohistochemical analysis of dog mammary adipose tissue, staining Perilipin-1 with ab3526.

      Tissue was fixed with formaldehyde and blocked with 1% blocking solution for 15 minutes at room temperature; antigen retrieval was by heat mediation in Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/200 in BSA in TBS) for 30 minutes. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (64)

    Publishing research using ab3526? Please let us know so that we can cite the reference in this datasheet.

    ab3526 has been referenced in 64 publications.

    • Yang Y  et al. O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun 11:181 (2020). PubMed: 31924761
    • Lu C  et al. Long non-coding RNA ARAP1-AS1 accelerates cell proliferation and migration in breast cancer through miR-2110/HDAC2/PLIN1 axis. Biosci Rep 40:N/A (2020). PubMed: 32110804
    • Rodriguez BL  et al. A tissue engineering approach for repairing craniofacial volumetric muscle loss in a sheep following a 2, 4, and 6-month recovery. PLoS One 15:e0239152 (2020). PubMed: 32956427
    • Xu Q  et al. Adipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactation1. J Anim Sci 97:2837-2849 (2019). PubMed: 31267132
    • Lynn EG  et al. 4-Phenylbutyrate protects against atherosclerotic lesion growth by increasing the expression of HSP25 in macrophages and in the circulation of Apoe-/- mice. FASEB J 33:8406-8422 (2019). PubMed: 30964709
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 13 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Perilipin-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (vertebrae bodies)
    Permeabilization
    Yes - trixton X-100
    Specification
    vertebrae bodies
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    10%NBF
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 21 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Perilipin-1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    Yes - Triton
    Specification
    Bone
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Tina Yuan

    Verified customer

    Submitted Sep 30 2019

    Immunohistochemistry (Resin sections) abreview for Anti-Perilipin-1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Human Tissue sections (adipocytes)
    Specification
    adipocytes
    Read More

    Dr. M Malatesta

    Verified customer

    Submitted Dec 01 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Perilipin-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (fat tissue)
    Permeabilization
    No
    Specification
    fat tissue
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 14 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Perilipin-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 27 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Perilipin-1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 10 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Perilipin-1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 10 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Perilipin-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cow Tissue sections (adipose)
    Antigen retrieval step
    Other
    Permeabilization
    Yes - 30 min 1x PBS 0.5% tritron buffer
    Specification
    adipose
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 06 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Perilipin-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Mammary Adipose Tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9 EDTA
    Permeabilization
    No
    Specification
    Mammary Adipose Tissue
    Blocking step
    DAKO PB as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 30 2012

    Question

    Thanks! I want to verify that the Expiration Date was written incorrectly. It should be March 27 2013, correct?  

    Read More

    Abcam community

    Verified customer

    Asked on Nov 27 2012

    Answer

    Thank you for your reply.

    Ypu are correct. The expiration is 27 March 2013.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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    Abcam Scientific Support

    Answered on Nov 27 2012

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