Immunohistochemistry (Resin sections) abreview for Anti-Perilipin-1 antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Resin sections)
Sample
Human Tissue sections (adipocytes)
Specification
adipocytes

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS Tween BSA
Dilution
1/5

Secondary antibody

Dilution
1/5
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L)-Jackson ImmunoResearch
Host species: Goat
Clonality: Polyclonal
Conjugation: Gold 12nm

Additional data

Additional Notes
The antibody has been tested at transmission electron microscopy in human adipocytes fixed with paraformaldehyde, trated with NH4Cl to block free aldehyde groups, dehydrated with graded concentrations of ethanol and embedded in acrylic(either LRWhite or Lowicryl K4M) resins. Embedding in Lowicryl K4M resin excluded thermic denaturation of the antigen since the whole processing occurs at low temperature. The antibody was tested diluted (from 1:20 to 1:5) as well as undiluted, but the result was always the same: an evident signal on chromatin (Ch) in the nuclei (N), some signal in the cytoplasm, a negligible signal around lipid droplets (L).
Abcam response
Thank you so much for testing this antibody in IHC-Resin. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this application. The customer received a discount on a future antibody purchase for testing this product. We welcome additional feedback from other customers as well.

Dr. M Malatesta

Verified customer

Submitted Dec 01 2017

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