Overview

  • Product name
  • Description
    Goat polyclonal to Perilipin-1
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Perilipin-1 aa 488-499 (internal sequence).
    Sequence:

    C-PREKPKRRVSDS

  • Positive control
    • WB: Human Adipose tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab60269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
ICC/IF Use at an assay dependent dilution. PubMed: 20335253

Target

  • Function
    Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
  • Tissue specificity
    Adipocytes.
  • Sequence similarities
    Belongs to the perilipin family.
  • Post-translational
    modifications
    Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
  • Cellular localization
    Lipid droplet. Lipid droplet surface-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lipid droplet associated protein antibody
    • Lipid droplet-associated protein antibody
    • PERI antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin-1 antibody
    • PerilipinA antibody
    • PLIN antibody
    • PLIN1 antibody
    • PLIN1_HUMAN antibody
    see all

Images

  • Anti-Perilipin-1 antibody (ab60269) at 0.01 µg/ml + Human adipose lysate (35µg protein in RIPA buffer)

    Predicted band size: 56 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:
  • Rodríguez-Castelán J  et al. Hypothyroidism Reduces the Size of Ovarian Follicles and Promotes Hypertrophy of Periovarian Fat with Infiltration of Macrophages in Adult Rabbits. Biomed Res Int 2017:3795950 (2017). Read more (PubMed: 28133606) »
  • Cheung W  et al. Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication. J Virol 84:6782-98 (2010). ICC/IF ; Rhesus monkey . Read more (PubMed: 20335253) »
See all 2 Publications for this product

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