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Cardiovascular Lipids / Lipoproteins Adipose Related Lipid Droplet Protein
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RecombinantRabMAb

Recombinant Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (4)

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Western blot - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)
  • Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3753(2)] to Perilipin-1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-Perilipin-1 antibody [EPR3753(2)]
    See all Perilipin-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3753(2)] to Perilipin-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: MouseDoes not react with: Rat
  • Immunogen

    Synthetic peptide within Human Perilipin-1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O60240

  • Positive control

    • Fetal heart, adipose tissue and feral liver lysates, HepG2 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3753(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Lipid Droplet Protein
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-Perilipin-1 antibody [EPR3753(2)] - BSA and Azide free (ab249727)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Perilipin-1 protein (ab112340)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
    • Tissue specificity

      Adipocytes.
    • Sequence similarities

      Belongs to the perilipin family.
    • Post-translational
      modifications

      Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
    • Cellular localization

      Lipid droplet. Lipid droplet surface-associated.
    • Target information above from: UniProt accession O60240 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5346 Human
      • Entrez Gene: 103968 Mouse
      • Omim: 170290 Human
      • SwissProt: O60240 Human
      • SwissProt: Q8CGN5 Mouse
      • Unigene: 103253 Human
      • Unigene: 254917 Mouse
      • Alternative names

        • Lipid droplet associated protein antibody
        • Lipid droplet-associated protein antibody
        • PERI antibody
        • Perilipin A antibody
        • Perilipin antibody
        • Perilipin-1 antibody
        • PerilipinA antibody
        • PLIN antibody
        • PLIN1 antibody
        • PLIN1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)
        Western blot - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)
        Lane 1 : Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907) at 1/2000 dilution
        Lanes 2-3 : Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907) at 1/10000 dilution

        Lane 1 : Fetal heart
        Lane 2 : Human adipose
        Lane 3 : Fetal liver

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 62 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)
        Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)

        Immunofluorescent analysis of HepG2 cells labeling Perilipin-1 with ab172907 at 1/100 (red) and counterstained with DAPI (blue).

      • Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)
        Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (4)

      Publishing research using ab172907? Please let us know so that we can cite the reference in this datasheet.

      ab172907 has been referenced in 4 publications.

      • Zhou Q  et al. Differentially expressed proteins identified by TMT proteomics analysis in bone marrow microenvironment of osteoporotic patients. Osteoporos Int 30:1089-1098 (2019). PubMed: 30739146
      • Li J  et al. Adipose HuR protects against diet-induced obesity and insulin resistance. Nat Commun 10:2375 (2019). PubMed: 31147543
      • Howie D  et al. A Novel Role for Triglyceride Metabolism in Foxp3 Expression. Front Immunol 10:1860 (2019). PubMed: 31456800
      • Li CJ  et al. Long noncoding RNA Bmncr regulates mesenchymal stem cell fate during skeletal aging. J Clin Invest 128:5251-5266 (2018). PubMed: 30352426

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Perilipin-1 antibody [EPR3753(2)]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Liver
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 10 2020

      Western blot abreview for Anti-Perilipin-1 antibody [EPR3753(2)]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (adipose tissue)
      Gel Running Conditions
      Reduced Denaturing (ReducedDenaturing(WES simple western))
      Loading amount
      0.75 µg
      Specification
      adipose tissue
      Read More

      Helle Zibrandtsen

      Verified customer

      Submitted Aug 15 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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