Recombinant Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Perilipin-1 antibody [EPR3753(2)]
See all Perilipin-1 primary antibodies -
DescriptionRabbit monoclonal [EPR3753(2)] to Perilipin-1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Synthetic peptide within Human Perilipin-1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O60240 -
Positive control
- Fetal heart, adipose tissue and feral liver lysates, HepG2 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3753(2)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab172907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 62 kDa. | |
| ICC/IF | 1/100 - 1/250. |
Target
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FunctionModulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
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Tissue specificityAdipocytes.
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Sequence similaritiesBelongs to the perilipin family.
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Post-translational
modificationsMajor cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. -
Cellular localizationLipid droplet. Lipid droplet surface-associated.
- Information by UniProt
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Database links
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- Unigene: 103253 Human
- Unigene: 254917 Mouse
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Alternative names
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)](https://www.abcam.com/ps/products/172/ab172907/Images/ab172907-195512-Immunofluorescent-staining-of-HepG2-cells-using-antiPerilipin-RabMAb-red-DAPI-nuclear-staining-blue.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)Immunofluorescent analysis of HepG2 cells labeling Perilipin-1 with ab172907 at 1/100 (red) and counterstained with DAPI (blue).
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![Western blot - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)](https://www.abcam.com/ps/products/172/ab172907/Images/ab172907-195513-8006WB2.jpg)
Western blot - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)Lane 1 : Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907) at 1/2000 dilution
Lanes 2-3 : Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907) at 1/10000 dilution
Lane 1 : Fetal heart
Lane 2 : Human adipose
Lane 3 : Fetal liver
Lysates/proteins at 10 µg per lane.
Predicted band size: 62 kDa
Datasheets and documents
References
This product has been referenced in:
- Zhou Q et al. Differentially expressed proteins identified by TMT proteomics analysis in bone marrow microenvironment of osteoporotic patients. Osteoporos Int 30:1089-1098 (2019). Read more (PubMed: 30739146) »
