Recombinant Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)
![Western blot - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)](https://www.abcam.com/ps/products/172/ab172907/Images/ab172907-195513-anti-perilipin-1-antibody-epr37532-western-blot.jpg "Western blot - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3753(2)] to Perilipin-1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Perilipin-1 antibody [EPR3753(2)]
See all Perilipin-1 primary antibodies -
Description
Rabbit monoclonal [EPR3753(2)] to Perilipin-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
Does not react with:
Rat -
Immunogen
Synthetic peptide within Human Perilipin-1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O60240 -
Positive control
- Fetal heart, adipose tissue and feral liver lysates, HepG2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3753(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
|
| ICC/IF | (1) |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 62 kDa. |
|
ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for IHC-P or IP.
Target
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Function
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. -
Tissue specificity
Adipocytes. -
Sequence similarities
Belongs to the perilipin family. -
Post-translational
modificationsMajor cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. -
Cellular localization
Lipid droplet. Lipid droplet surface-associated. - Information by UniProt
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Database links
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- Unigene: 103253 Human
- Unigene: 254917 Mouse
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Alternative names
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
Images
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Western blot - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)Lane 1 : Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907) at 1/2000 dilution
Lanes 2-3 : Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907) at 1/10000 dilution
Lane 1 : Fetal heart
Lane 2 : Human adipose
Lane 3 : Fetal liver
Lysates/proteins at 10 µg per lane.
Predicted band size: 62 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)](https://www.abcam.com/ps/products/172/ab172907/Images/ab172907-195512-anti-perilipin-1-antibody-epr37532-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)Immunofluorescent analysis of HepG2 cells labeling Perilipin-1 with ab172907 at 1/100 (red) and counterstained with DAPI (blue).
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![Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)](https://www.abcam.com/ps/products/172/ab172907/Images/ab172907-3-benefits-of-recombinant-antibodies.png)
Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab172907 has been referenced in 5 publications.
- Maldonado M et al. The consequences of a high-calorie diet background before calorie restriction on skeletal muscles in a mouse model. Aging (Albany NY) 13:16834-16858 (2021). PubMed: 34166224
- Zhou Q et al. Differentially expressed proteins identified by TMT proteomics analysis in bone marrow microenvironment of osteoporotic patients. Osteoporos Int 30:1089-1098 (2019). PubMed: 30739146
- Li J et al. Adipose HuR protects against diet-induced obesity and insulin resistance. Nat Commun 10:2375 (2019). PubMed: 31147543
- Howie D et al. A Novel Role for Triglyceride Metabolism in Foxp3 Expression. Front Immunol 10:1860 (2019). PubMed: 31456800
- Li CJ et al. Long noncoding RNA Bmncr regulates mesenchymal stem cell fate during skeletal aging. J Clin Invest 128:5251-5266 (2018). PubMed: 30352426
