Key features and details
- Rabbit polyclonal to Perilipin 3/TIP47
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Perilipin 3/TIP47 antibody
See all Perilipin 3/TIP47 primary antibodies
DescriptionRabbit polyclonal to Perilipin 3/TIP47
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- WB: U-86 MG cell lysate. IHC-P: Human small intestine tissue. ICC/IF: A431 cells.
Previously labelled as Perilipin 3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionRequired for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
Sequence similaritiesBelongs to the perilipin family.
Cellular localizationCytoplasm. Endosome membrane. Lipid droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
- Information by UniProt
- 47 kDa mannose 6-phosphate receptor-binding protein antibody
- 47 kDa MPR binding protein antibody
- 47 kDa MPR-binding protein antibody
Anti-Perilipin 3/TIP47 antibody (ab224344) at 1/100 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Predicted band size: 47 kDa
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Perilipin 3/TIP47 (green) using ab224344 (4 µg/ml) in ICC/IF.
Paraffin-embedded human small intestine tissue stained for Perilipin 3/TIP47 with ab224344 (1/200) in immunohistochemical analysis.
ab224344 has not yet been referenced specifically in any publications.