Key features and details
- Rabbit polyclonal to Perilipin 3/TIP47
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Perilipin 3/TIP47 antibody
See all Perilipin 3/TIP47 primary antibodies
DescriptionRabbit polyclonal to Perilipin 3/TIP47
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human Perilipin 3/TIP47 aa 75-125. The exact sequence is proprietary.
Database link: O60664
- IP: HEK-293T whole cell lysate.
ab245569 has not performed satisfactorily when used for WB of Perilipin 3/TIP47 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Perilipin 3/TIP47.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245569 was affinity purified using an epitope specific to Perilipin 3/TIP47 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRequired for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
Sequence similaritiesBelongs to the perilipin family.
Cellular localizationCytoplasm. Endosome membrane. Lipid droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
- Information by UniProt
- 47 kDa mannose 6-phosphate receptor-binding protein antibody
- 47 kDa MPR binding protein antibody
- 47 kDa MPR-binding protein antibody
Perilipin 3/TIP47 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245569 used for IP at 6 µg per reaction. For WB 1 µg/ml.
Lane 1: ab245569 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Chemiluminescence detection: 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245569 has not yet been referenced specifically in any publications.