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Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (ab150680)

  • Datasheet
  • SDS
  • Protocol Booklet
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ab150680 - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)
  • ab150680 - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)

Key features and details

  • Assay time: 45 min
  • Sample type: Tissue sections

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Overview

  • Product name

    Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)
  • Sample type

    Tissue sections
  • Assay time

    0h 45m
  • Product overview

    The Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) is intended for use in histological demonstration of lymphocytes and mucopolysaccharides. The staining pattern of the lymphocytes are helpful in making therapeutic decisions in established cases of lymphocytic leukemia. The PAS reaction in tissue sections is useful for the demonstration of mucopolysaccharides. PAS staining may also be used for the demonstration of fungal organisms in tissue sections.


    PAS staining protocol summary:
    - deparaffinize sections (if necessary) and hydrate
    - immerse slide in PAS solution for 5-10 min
    - rinse 4 times with water
    - immerse slide in Schiff's solution for 15-30 min and rinse
    - stain slide with Hematoxylin for 2-3 min  and rinse
    - incubate in Bluing reagent for 30 s and rinse
    - incubate in Light Green Solution for 2 min and rinse
    - dehydrate with alcohol, clear and mount


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation

    Fungal Organisms Magenta
    PAS Positive Material   Magenta
    Nuclei Black/Blue
    Other Tissue Components    Green/Blue

    Control Tissue: Kidney, Intestine, Liver.

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 100 tests
    Bluing Reagent 1 x 125ml
    Hematoxylin (Modified Mayer's Solution) 1 x 125ml
    Light Green Solution 1 x 125ml
    Periodic acid solution 1 x 250ml
    Schiff's solution 1 x 250ml
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • MUC
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MUC
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Counterstains and Special stains
    • PAS Stain

Associated products

    Images

    • ab150680 - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)
      ab150680 - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)

      ab150680 Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human kidney.

    • ab150680 - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)
      ab150680 - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)

      Staining of Intestine using ab150680 - Periodic Acid Schiff (PAS) Stain Kit.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (16)

    Publishing research using ab150680? Please let us know so that we can cite the reference in this datasheet.

    ab150680 has been referenced in 16 publications.

    • Khan HU  et al. Food additive "lauric acid" possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats. PeerJ 8:e8805 (2020). PubMed: 32266118
    • Tao S  et al. Original low birth weight deteriorates the hindgut epithelial barrier function in pigs at the growing stage. FASEB J 33:9897-9912 (2019). PubMed: 31170357
    • Ramli NSK  et al. Hormonal control of vas deferens fluid volume and aquaporin expression in rats. J Mol Histol 50:21-34 (2019). PubMed: 30430402
    • Heo JY & Im DS Anti-allergic effects of salvianolic acid A and tanshinone IIA from Salvia miltiorrhiza determined using in vivo and in vitro experiments. Int Immunopharmacol 67:69-77 (2019). PubMed: 30537633
    • Kim MJ & Im DS Suppressive effects of type I angiotensin receptor antagonists, candesartan and irbesartan on allergic asthma. Eur J Pharmacol 852:25-33 (2019). PubMed: 30797786
    View all Publications for this product

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