Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (ab150680)
- Datasheet
- References (14)
- Protocols
Overview
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Product name
Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) -
Sample type
Tissue sections -
Assay time
0h 45m -
Product overview
The Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) is intended for use in histological demonstration of lymphocytes and mucopolysaccharides. The staining pattern of the lymphocytes are helpful in making therapeutic decisions in established cases of lymphocytic leukemia. The PAS reaction in tissue sections is useful for the demonstration of mucopolysaccharides. PAS staining may also be used for the demonstration of fungal organisms in tissue sections.
PAS staining protocol summary:
- deparaffinize sections (if necessary) and hydrate
- immerse slide in PAS solution for 5-10 min
- rinse 4 times with water
- immerse slide in Schiff's solution for 15-30 min and rinse
- stain slide with Hematoxylin for 2-3 min and rinse
- incubate in Bluing reagent for 30 s and rinse
- incubate in Light Green Solution for 2 min and rinse
- dehydrate with alcohol, clear and mount
Other products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Notes
Staining Interpretation
Fungal Organisms Magenta PAS Positive Material Magenta Nuclei Black/Blue Other Tissue Components Green/Blue Control Tissue: Kidney, Intestine, Liver.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Bluing Reagent 1 x 125ml Hematoxylin (Modified Mayer's Solution) 1 x 125ml Light Green Solution 1 x 125ml Periodic acid solution 1 x 250ml Schiff's solution 1 x 250ml -
Research areas
Images
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ab150680 - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)ab150680 Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human kidney.
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ab150680 - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)Staining of Intestine using ab150680 - Periodic Acid Schiff (PAS) Stain Kit.
Protocols
References
This product has been referenced in:
- Ramli NSK et al. Hormonal control of vas deferens fluid volume and aquaporin expression in rats. J Mol Histol 50:21-34 (2019). Read more (PubMed: 30430402) »
- Heo JY & Im DS Anti-allergic effects of salvianolic acid A and tanshinone IIA from Salvia miltiorrhiza determined using in vivo and in vitro experiments. Int Immunopharmacol 67:69-77 (2019). Read more (PubMed: 30537633) »
