Overview

  • Product name
    Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)
  • Sample type
    Tissue sections
  • Assay time
    0h 45m
  • Product overview

    The Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) is intended for use in histological demonstration of lymphocytes and mucopolysaccharides. The staining pattern of the lymphocytes are helpful in making therapeutic decisions in established cases of lymphocytic leukemia. The PAS reaction in tissue sections is useful for the demonstration of mucopolysaccharides. PAS staining may also be used for the demonstration of fungal organisms in tissue sections.


    PAS staining protocol summary:
    - deparaffinize sections (if necessary) and hydrate
    - immerse slide in PAS solution for 5-10 min
    - rinse 4 times with water
    - immerse slide in Schiff's solution for 15-30 min and rinse
    - stain slide with Hematoxylin for 2-3 min  and rinse
    - incubate in Bluing reagent for 30 s and rinse
    - incubate in Light Green Solution for 2 min and rinse
    - dehydrate with alcohol, clear and mount


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation

    Fungal Organisms Magenta
    PAS Positive Material   Magenta
    Nuclei Black/Blue
    Other Tissue Components    Green/Blue

    Control Tissue: Kidney, Intestine, Liver.

Properties

Images

  • ab150680 Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human kidney.

  • Staining of Intestine using ab150680 - Periodic Acid Schiff (PAS) Stain Kit.

Protocols

References

This product has been referenced in:
  • Ramli NSK  et al. Hormonal control of vas deferens fluid volume and aquaporin expression in rats. J Mol Histol 50:21-34 (2019). Read more (PubMed: 30430402) »
  • Heo JY & Im DS Anti-allergic effects of salvianolic acid A and tanshinone IIA from Salvia miltiorrhiza determined using in vivo and in vitro experiments. Int Immunopharmacol 67:69-77 (2019). Read more (PubMed: 30537633) »
See all 13 Publications for this product

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