Overview

  • Product name
  • Description
    Rabbit polyclonal to Periostin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human Periostin (N terminal).

  • General notes

    Lots # GR310924-1, GR296166-4, and GR296166-5 are sold in lyophilised form. To reconstitute, add 100 µl of deionized water and let the lyophilised antibody dissolve completely. The resulting concentration is 0.5 mg/ml. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Properties

Applications

Our Abpromise guarantee covers the use of ab14041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 19079190
IHC-P 1/100 - 1/1000.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 100 kDa.
ELISA 1/100 - 1/1000.

Target

  • Function
    Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
  • Tissue specificity
    Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
  • Sequence similarities
    Contains 1 EMI domain.
    Contains 4 FAS1 domains.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fasciclin-I like antibody
    • MGC119510 antibody
    • MGC119511 antibody
    • OSF 2 antibody
    • OSF-2 antibody
    • OSF2 antibody
    • Osteoblast specific factor 2 (fasciclin I like) antibody
    • Osteoblast specific factor 2 antibody
    • Osteoblast specific factor antibody
    • Osteoblast-specific factor 2 antibody
    • PDLPOSTN antibody
    • Periodontal ligament specific periostin antibody
    • Periostin antibody
    • Periostin isoform thy2 antibody
    • Periostin isoform thy4 antibody
    • Periostin isoform thy6 antibody
    • Periostin isoform thy8 antibody
    • Periostin osteoblast specific factor antibody
    • PN antibody
    • POSTN antibody
    • POSTN_HUMAN antibody
    • RP11 412K4.1 antibody
    see all

Images

  • ab14041 at a 1/100 dilution staining Periostin in mouse dermis tissue by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized using 0.1% Triton. Blocked using 20% serum for 1 hour at 4°C. Secondary used at 1/200 polyclonal Donkey anti-rabbit conjugated to Alexa Fluor 555 (red). Counterstain (blue) DAPI.

    See Abreview

  • ab14041, staining Periostin in Human breast carcinoma tissue by Immunohistochemistry (Frozen sections).

    Sections were acetone-fixed and blocked in normal serum. The sections were incubated with the primary antibody (for 1 hour at room temperature), followed by incubation with an HRP-conjugated secondary antibody for additional 30 minutes at room temperature. Diaminobenzidine (DAB) was used as a chromogen resulting in brown staining of positive cells. The nuclei were counterstained in using hematoxylin.
  • ICC/IF image of ab14041 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab14041 staining Periostin in Mouse PDL tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, permeablized with Triton-X, blocked with 5% serum for 60 minutes at 25°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200) at 4°C for 16 hours. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody. HRP-streptavidin and DAB for detection

    See Abreview

References

This product has been referenced in:
  • Dias DO  et al. Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury. Cell 173:153-165.e22 (2018). Read more (PubMed: 29502968) »
  • Shimoyama Y  et al. Periostin attenuates tumor growth by inducing apoptosis in colitis-related colorectal cancer. Oncotarget 9:20008-20017 (2018). Read more (PubMed: 29731999) »

See all 91 Publications for this product

Customer reviews and Q&As

The selling size of ab14041 is 50ugand in lyophilized form. We recommend to reconstitute the product in 0.05ml deionized water. This would result in 1ug/ul (50ug in 0.05ml water).

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (PDL)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Tris Antigen Retrieval Solution
Permeabilization
Yes - Triton X
Specification
PDL
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Tina Yuan

Verified customer

Submitted Jan 25 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (tooth-PDL)
Permeabilization
Yes - Triton X
Specification
tooth-PDL
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Dr. Tina Yuan

Verified customer

Submitted Jan 04 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Jaculus jaculus Tissue sections (Metatarsals)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid based
Permeabilization
No
Specification
Metatarsals
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Fixative
Paraformaldehyde
Username

Haydee Gutierrez

Verified customer

Submitted Jun 08 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Periosteum Sheep Tibia)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinas K - Ready to use (Dako - S3020)
Permeabilization
No
Specification
Periosteum Sheep Tibia
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 11 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 07 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 27 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Blockace as blocking agent for 10 minute(s) · Concentration: 4% · Temperature: 4°C
Sample
Dog Tissue sections (Skin)
Specification
Skin
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 19 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
None
Sample
Dog Tissue sections (Skin)
Specification
Skin
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 19 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrition buffer
Sample
Mouse Tissue sections (mouse embryo)
Specification
mouse embryo
Permeabilization
Yes - 1% triton x-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 02 2014

1-10 of 28 Abreviews or Q&A

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