Overview

  • Product name
  • Description
    Rabbit polyclonal to Periostin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human Periostin (N terminal).

  • General notes

    Lots # GR310924-1, GR296166-4, and GR296166-5 are sold in lyophilized form. To reconstitute, add 100 µL of deionized water and let the lyophilized antibody dissolve completely. The resulting concentration is 0.5 mg/mL. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Properties

Applications

Our Abpromise guarantee covers the use of ab14041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 19079190
IHC-P 1/100 - 1/1000.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 100 kDa.
ELISA 1/100 - 1/1000.

Target

  • Function
    Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
  • Tissue specificity
    Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
  • Sequence similarities
    Contains 1 EMI domain.
    Contains 4 FAS1 domains.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fasciclin-I like antibody
    • MGC119510 antibody
    • MGC119511 antibody
    • OSF 2 antibody
    • OSF-2 antibody
    • OSF2 antibody
    • Osteoblast specific factor 2 (fasciclin I like) antibody
    • Osteoblast specific factor 2 antibody
    • Osteoblast specific factor antibody
    • Osteoblast-specific factor 2 antibody
    • PDLPOSTN antibody
    • Periodontal ligament specific periostin antibody
    • Periostin antibody
    • Periostin isoform thy2 antibody
    • Periostin isoform thy4 antibody
    • Periostin isoform thy6 antibody
    • Periostin isoform thy8 antibody
    • Periostin osteoblast specific factor antibody
    • PN antibody
    • POSTN antibody
    • POSTN_HUMAN antibody
    • RP11 412K4.1 antibody
    see all

Images

  • The periostin staining in prostate cancer and paired non-tumorous prostate tissues (TMA from JHU, 140 cases).

    (A) The pathologists’ criteria for IHC scoring.(a) 0, undetectable; (b)1+, weak staining; (c) 2+, medium staining; (d) 3+, strong staining. Periostin was mainly stained in peritumoral stroma.

  • Phenotypic analysis of mouse hearts with combined loss of Tbx18 and Tbx2 in the epicardium at E18.5.

    (B) Immunofluorescence analysis of ACTA2 and TAGLN expression shows normal differentiation of coronary SMCs and their localization to coronary arteries (CAr). Capillary density, although not quantified, appears unaffected by the loss of Tbx18, Tbx2 or both genes in the epicardium as visualized by immunofluorescence against EMCN. The presence of POSTN in the myocardium confirms the formation of cardiac fibroblasts from epicardial cells in all mutants. Two specimens per genotype were analyzed.

  • ab14041, staining Periostin in human breast carcinoma tissue by Immunohistochemistry (Frozen sections).

    Sections were acetone-fixed and blocked in normal serum. The sections were incubated with the primary antibody (for 1 hour at room temperature), followed by incubation with an HRP-conjugated secondary antibody for additional 30 minutes at room temperature. Diaminobenzidine (DAB) was used as a chromogen resulting in brown staining of positive cells. The nuclei were counterstained in using hematoxylin.

  • All lanes : Anti-Periostin antibody (ab14041)

    Lanes 1-2 : Normoxic Mouse lung lysate
    Lanes 3-4 : Hyperoxic Mouse lung lysate
    Lanes 5-6 : Normoxic Mouse lung lysate from periostin KO mouse
    Lanes 7-8 : Hypertoxic Mouse lung lysate from periostin KO mouse

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 100 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 80-90 kDa (possible cleavage fragment)



    Results from two normoxic wild-type mice and two hyperoxic wild-type mice are shown here; lysates from normoxic and hyperoxic periostin null mice are added for comparison.

    The full-length periostin protein is 90 kD in size, but there are also products of splice variants. Hyperoxic exposure increased periostin protein abundance over two-fold.

  • ab14041 at a 1/100 dilution staining Periostin in mouse dermis tissue by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized using 0.1% Triton. Blocked using 20% serum for 1 hour at 4°C. Secondary used at 1/200 polyclonal Donkey anti-rabbit conjugated to Alexa Fluor®555 (red). Counterstain (blue) DAPI.

    See Abreview

  • ab14041 staining Periostin in mouse PDL tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections).

    Tissue samples were fixed by perfusion with formaldehyde, permeablized with Triton-X, blocked with 5% serum for 60 minutes at 25°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200) at 4°C for 16 hours. A biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody. HRP-streptavidin and DAB for detection.

    See Abreview

  • ICC/IF image of ab14041 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Kang Y  et al. Periostin serves an important role in the pathogenesis of oral squamous cell carcinoma. Oncol Lett 17:1292-1298 (2019). Read more (PubMed: 30655897) »
  • Kamimoto H  et al. Relaxin 2 carried by magnetically directed liposomes accelerates rat midpalatal suture expansion and subsequent new bone formation. Bone Rep 10:100202 (2019). Read more (PubMed: 30937342) »
See all 112 Publications for this product

Customer reviews and Q&As

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1-10 of 19 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TBST
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 03 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Jorge Guerrero

Verified customer

Submitted Jun 20 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (tooth)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vector antigen unmask solution
Permeabilization
Yes - triton
Specification
tooth
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jun 16 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (periodontal ligament)
Specification
periodontal ligament
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jun 16 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (PDL)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Tris Antigen Retrieval Solution
Permeabilization
Yes - Triton X
Specification
PDL
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jan 25 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (tooth-PDL)
Permeabilization
Yes - Triton X
Specification
tooth-PDL
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jan 04 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Jaculus jaculus Tissue sections (Metatarsals)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid based
Permeabilization
No
Specification
Metatarsals
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Fixative
Paraformaldehyde

Haydee Gutierrez

Verified customer

Submitted Jun 08 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Periosteum Sheep Tibia)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinas K - Ready to use (Dako - S3020)
Permeabilization
No
Specification
Periosteum Sheep Tibia
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 11 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 07 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 27 2015

1-10 of 19 Abreviews

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