Anti-Periostin antibody (ab14041)
Key features and details
- Rabbit polyclonal to Periostin
- Suitable for: ELISA, ICC/IF, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Periostin antibody
See all Periostin primary antibodies -
Description
Rabbit polyclonal to Periostin -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human Periostin (N terminal).
-
Positive control
- IHC-P: Human colon and normal skin tissues; Prostate cancer tissues. ICC/IF: HeLa cells.
-
General notes
Lots # GR310924-1, GR296166-4, and GR296166-5 are sold in lyophilized form. To reconstitute, add 100 µL of deionized water and let the lyophilized antibody dissolve completely. The resulting concentration is 0.5 mg/mL. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 0.58% Sodium chloride -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
ICC/IF | (3) |
Use at an assay dependent concentration.
|
IHC-Fr | (6) |
Use at an assay dependent concentration. PubMed: 19079190
|
IHC-P | (8) |
1/100 - 1/1000.
|
Notes |
---|
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 19079190 |
IHC-P
1/100 - 1/1000. |
Target
-
Function
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
Tissue specificity
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
Database links
- Entrez Gene: 10631 Human
- Entrez Gene: 50706 Mouse
- Entrez Gene: 361945 Rat
- Omim: 608777 Human
- SwissProt: Q15063 Human
- SwissProt: Q62009 Mouse
- Unigene: 136348 Human
- Unigene: 721018 Human
see all -
Alternative names
- Fasciclin-I like antibody
- MGC119510 antibody
- MGC119511 antibody
see all
Images
-
Immunohistochemistry analysis of formalin-fixed paraffin-embedded human colon tissue section labeling Periostin with ab14041.
-
Immunohistochemistry analysis of formalin-fixed paraffin-embedded human normal skin tissue sectionlabeling Periostin with ab14041.
-
The periostin staining in prostate cancer and paired non-tumorous prostate tissues (TMA from JHU, 140 cases).
(A) The pathologists’ criteria for IHC scoring.(a) 0, undetectable; (b)1+, weak staining; (c) 2+, medium staining; (d) 3+, strong staining. Periostin was mainly stained in peritumoral stroma.
-
ICC/IF image of ab14041 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (193)
ab14041 has been referenced in 193 publications.
- Daghrery A et al. Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues. Bioact Mater 19:268-281 (2023). PubMed: 35574052
- Fleming Martinez AK et al. Ym1+ macrophages orchestrate fibrosis, lesion growth, and progression during development of murine pancreatic cancer. iScience 25:104327 (2022). PubMed: 35602933
- Jang SY et al. Therapeutic Potential of Targeting Periostin in the Treatment of Graves' Orbitopathy. Front Endocrinol (Lausanne) 13:900791 (2022). PubMed: 35707463
- Miyai K et al. Prognostic impact of stromal periostin expression in upper urinary tract urothelial carcinoma. BMC Cancer 22:787 (2022). PubMed: 35850759
- Tsukasaki M et al. Periosteal stem cells control growth plate stem cells during postnatal skeletal growth. Nat Commun 13:4166 (2022). PubMed: 35851381