Anti-Periostin antibody (ab231130)
Key features and details
- Rabbit polyclonal to Periostin
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Periostin antibody
See all Periostin primary antibodies -
Description
Rabbit polyclonal to Periostin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Rat Periostin aa 1-200. N-terminal tag. Expressed in E.coli.
Database link: D3ZAF5 -
Positive control
- WB: Recombinant rat Periostin protein; Rat lung lysate and serum. IHC-P: Rat liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 93 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 93 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
Tissue specificity
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 10631 Human
- Entrez Gene: 50706 Mouse
- Entrez Gene: 361945 Rat
- Omim: 608777 Human
- SwissProt: Q15063 Human
- SwissProt: Q62009 Mouse
- Unigene: 136348 Human
- Unigene: 721018 Human
see all -
Alternative names
- Fasciclin-I like antibody
- MGC119510 antibody
- MGC119511 antibody
see all
Images
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Anti-Periostin antibody (ab231130) at 2 µg/ml + Recombinant rat Periostin protein.
Developed using the ECL technique.
Predicted band size: 93 kDa -
Anti-Periostin antibody (ab231130) at 2 µg/ml + Rat lung tissue lysate.
Developed using the ECL technique.
Predicted band size: 93 kDa -
Anti-Periostin antibody (ab231130) at 2 µg/ml + Rat serum.
Developed using the ECL technique.
Predicted band size: 93 kDa -
Formalin-fixed, paraffin-embedded rat liver tissue stained for Periostin using ab231130 at 20 μg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231130 has not yet been referenced specifically in any publications.