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    periostin-antibody-ab79946.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Focal Adhesions
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Anti-Periostin antibody (ab79946)

  • Datasheet
Reviews (1)Q&A (1)References (7)

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Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab79946)
  • Western blot - Anti-Periostin antibody (ab79946)
  • Immunocytochemistry/ Immunofluorescence - Anti-Periostin antibody (ab79946)

Key features and details

  • Rabbit polyclonal to Periostin
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Primary
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Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
Protein
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Recombinant Human Periostin protein (ab203522)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Periostin antibody
    See all Periostin primary antibodies
  • Description

    Rabbit polyclonal to Periostin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Periostin aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90847)

  • Positive control

    • This antibody gave a positive signal in WB in Human breast tumour tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast fibroadenocarcinoma.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Cardiomyocyte precursors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Periostin protein (ab203522)

Applications

Our Abpromise guarantee covers the use of ab79946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80, 86, 86, 93 kDa (predicted molecular weight: 93 kDa). This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2,3 & 4 of Human Periostin.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
  • Tissue specificity

    Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
  • Sequence similarities

    Contains 1 EMI domain.
    Contains 4 FAS1 domains.
  • Post-translational
    modifications

    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession Q15063 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10631 Human
    • Entrez Gene: 50706 Mouse
    • Omim: 608777 Human
    • SwissProt: Q15063 Human
    • SwissProt: Q62009 Mouse
    • Unigene: 136348 Human
    • Unigene: 721018 Human
    • Unigene: 236067 Mouse
    • Alternative names

      • Fasciclin-I like antibody
      • MGC119510 antibody
      • MGC119511 antibody
      • OSF 2 antibody
      • OSF-2 antibody
      • OSF2 antibody
      • Osteoblast specific factor 2 (fasciclin I like) antibody
      • Osteoblast specific factor 2 antibody
      • Osteoblast specific factor antibody
      • Osteoblast-specific factor 2 antibody
      • PDLPOSTN antibody
      • Periodontal ligament specific periostin antibody
      • Periostin antibody
      • Periostin isoform thy2 antibody
      • Periostin isoform thy4 antibody
      • Periostin isoform thy6 antibody
      • Periostin isoform thy8 antibody
      • Periostin osteoblast specific factor antibody
      • PN antibody
      • POSTN antibody
      • POSTN_HUMAN antibody
      • RP11 412K4.1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab79946)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab79946)

      IHC image of Periostin staining in Human breast fibroadenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79946, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-Periostin antibody (ab79946)
      Western blot - Anti-Periostin antibody (ab79946)
      Anti-Periostin antibody (ab79946) at 1 µg/ml (blocked with 3% milk) + Breast (Human) Cytoplasmic Lysate - tumor tissue (ab30085) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 93 kDa
      Observed band size: 80,80,86,87,93 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2,3 & 4 of Human Periostin. The predicted molecular weights of isoforms 1,2,3 & 4 are 93, kDa, 87 kDa, 87 kDa and 83 kDa respectively.
    • Immunocytochemistry/ Immunofluorescence - Anti-Periostin antibody (ab79946)
      Immunocytochemistry/ Immunofluorescence - Anti-Periostin antibody (ab79946)
      ICC/IF image of ab79946 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79946, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (7)

    Publishing research using ab79946? Please let us know so that we can cite the reference in this datasheet.

    ab79946 has been referenced in 7 publications.

    • Zhang Q  et al. Cancer-associated stroma significantly contributes to the mesenchymal subtype signature of serous ovarian cancer. Gynecol Oncol N/A:N/A (2018). PubMed: 30448260
    • Wei Y  et al. Excessive periostin expression and Th2 response in patients with nasal polyps: association with asthma. J Thorac Dis 10:6585-6597 (2018). PubMed: 30746204
    • Meng X  et al. Periostin has a protective role in melatonin-induced cell apoptosis by inhibiting the eIF2a-ATF4 pathway in human osteoblasts. Int J Mol Med 41:1003-1012 (2018). PubMed: 29207036
    • Nagaraju CK  et al. Global fibroblast activation throughout the left ventricle but localized fibrosis after myocardial infarction. Sci Rep 7:10801 (2017). PubMed: 28883544
    • Sverrild A  et al. Eosinophilic airway inflammation in asthmatic patients is associated with an altered airway microbiome. J Allergy Clin Immunol 140:407-417.e11 (2017). PubMed: 28042058
    • Guo X  et al. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. Oncotarget 7:80521-80542 (2016). WB ; Human . PubMed: 27602954
    • Kong P  et al. Lack of specificity of fibroblast-specific protein 1 in cardiac remodeling and fibrosis. Am J Physiol Heart Circ Physiol 305:H1363-72 (2013). IHC ; Mouse . PubMed: 23997102

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Good morning, We have ordered an Abcam antibody for periostin [ab79946. What is the concentration of the antibody? What concentration do you suggest for IHC? Should we reconstitute the antibody? Is antigen retrienval step with citrate recommened? Thanks in advance

    Read More

    Abcam community

    Verified customer

    Asked on Sep 27 2012

    Answer

    Thank you for contacting us.

    The lot of this antibody that you will receive is 0.9 mg/ml. Please note that another lot in our inventory has a concentration of 0.1 mg/ml. The concentration will vary from one lot to another.

    For IHC, we recommend a concentration of 5ug/ml, which for the lot your receive will be a dilution of 1/180. (A dilution of 1/200 will be fine). The antibody is already in solution. There is no need to reconstitute.

    A heat -mediated antigen retrieval is recommended. Users have reported success with a citrate buffer, pH 6.0, and also an EDTA buffer, pH 9.0. Sections were incubated with either of these retrieval buffers for 20 minutes at 95-100C.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Sep 27 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Periostin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (testis)
    Specification
    testis
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 20 min, 100C
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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