Overview

  • Product name

    Anti-Periostin antibody [EPR19934] - BSA and Azide free
    See all Periostin primary antibodies
  • Description

    Rabbit monoclonal [EPR19934] to Periostin - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes a different epitope of Periostin compared to ab219057.
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Periostin aa 800 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15063

  • Positive control

    • WB: Human fetal heart, fetal kidney and breast cancer lysates. IHC-P: Human colon and lung cancer tissues. IP: Human breast cancer lysate.
  • General notes

    Ab223194 is the carrier-free version of ab219056. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab223194 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab223194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).

Target

  • Function

    Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
  • Tissue specificity

    Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
  • Sequence similarities

    Contains 1 EMI domain.
    Contains 4 FAS1 domains.
  • Post-translational
    modifications

    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fasciclin-I like antibody
    • MGC119510 antibody
    • MGC119511 antibody
    • OSF 2 antibody
    • OSF-2 antibody
    • OSF2 antibody
    • Osteoblast specific factor 2 (fasciclin I like) antibody
    • Osteoblast specific factor 2 antibody
    • Osteoblast specific factor antibody
    • Osteoblast-specific factor 2 antibody
    • PDLPOSTN antibody
    • Periodontal ligament specific periostin antibody
    • Periostin antibody
    • Periostin isoform thy2 antibody
    • Periostin isoform thy4 antibody
    • Periostin isoform thy6 antibody
    • Periostin isoform thy8 antibody
    • Periostin osteoblast specific factor antibody
    • PN antibody
    • POSTN antibody
    • POSTN_HUMAN antibody
    • RP11 412K4.1 antibody
    see all

References

ab223194 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223194.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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