Overview

  • Product name
  • Description
    Chicken polyclonal to Peripherin
  • Host species
    Chicken
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    Recombinant full length protein corresponding to Rat Peripherin.

  • Positive control
    • WB: Rat, mouse, pig and cow spinal cord lysate; SH-SY5Y and PC-12 cell lysate. ICC/IF: PC-12 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab24524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/2000.
WB 1/20000. Detects a band of approximately 57 kDa.

Target

Images

  • All lanes : Anti-Peripherin antibody (ab24524) at 1/10000 dilution

    Lane 2 : Rat spinal cord lysate
    Lane 3 : Mouse spinal cord lysate
    Lane 4 : Pig spinal cord lysate
    Lane 5 : Cow spinal cord lysate
    Lane 6 : SH-S5Y5 (Human neuroblastoma cell line from bone marrow) cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
  • Immunofluorescent analysis of mixed fibroblast and PC-12 (rat adrenal gland pheochromocytoma cell line) pheochromocytoma cell culture stained for Peripherin using ab24524 (green) at a 1/5000 dilution and co-stained with rabbit anti-vimentin (red) at a 1/2000 dilution. The blue is DAPI staining of nuclear DNA.

    ab24524 reveals cytoplasmic filamentous staining in the small PC-12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.

References

This product has been referenced in:
  • Roy A  et al. Anandamide modulates carotid sinus nerve afferent activity via TRPV1 receptors increasing responses to heat. J Appl Physiol 112:212-24 (2012). IHC-FoFr ; Rat . Read more (PubMed: 21903882) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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