Product nameAnti-Peripherin antibody
See all Peripherin primary antibodies
DescriptionChicken polyclonal to Peripherin
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Pig
Recombinant full length protein corresponding to Rat Peripherin.
- WB: Rat, mouse, pig and cow spinal cord lysate; SH-SY5Y and PC-12 cell lysate. ICC/IF: PC-12 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.065% Sodium azide
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab24524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/20000. Detects a band of approximately 57 kDa.|
FunctionClass-III neuronal intermediate filament protein.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- NEF 4 antibody
- NEF4 antibody
- Neurofilament 4 (57kD) antibody
All lanes : Anti-Peripherin antibody (ab24524) at 1/10000 dilution
Lane 2 : Rat spinal cord lysate
Lane 3 : Mouse spinal cord lysate
Lane 4 : Pig spinal cord lysate
Lane 5 : Cow spinal cord lysate
Lane 6 : SH-S5Y5 (Human neuroblastoma cell line from bone marrow) cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Immunofluorescent analysis of mixed fibroblast and PC-12 (rat adrenal gland pheochromocytoma cell line) pheochromocytoma cell culture stained for Peripherin using ab24524 (green) at a 1/5000 dilution and co-stained with rabbit anti-vimentin (red) at a 1/2000 dilution. The blue is DAPI staining of nuclear DNA.
ab24524 reveals cytoplasmic filamentous staining in the small PC-12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.