Product nameAnti-Peripherin antibody
See all Peripherin primary antibodies
DescriptionChicken polyclonal to Peripherin
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant protein expressed in bacteria.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
(10mM isotonic 0.9% w/v), pH 7.2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab39374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/50 - 1/100.|
FunctionClass-III neuronal intermediate filament protein.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- NEF 4 antibody
- NEF4 antibody
- Neurofilament 4 (57kD) antibody
ab39374 staining Peripherin in Human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/250 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor® 488-conjugated Goat anti-chicken IgY polyclonal (1/500) was used as the secondary antibody.
Ab39374 staining human peripherin in neonatal rat cortical neurons cultured for 14-21 days by ICC/IF.
ab39374 at a 1/100 dilution staining Peripherin in rat brain tissue (cerebellum) by Immunohistochemistry (PFA fixed, frozen sections), incubated for 12 hours at 4°C. Permeabilized using 0.3% Triton. Blocked with 10% donkey serum for 1 hour at 22°C. Secondary used at 1/500 polyclonal Goat Vector BA 9010 conjugated to biotin. Biotin detected with streptavidin Alexa Fluor 594.
The image shows a climbing fiber in the cerebellar molecular layer.
This product has been referenced in:
- Hill RZ et al. The signaling lipid sphingosine 1-phosphate regulates mechanical pain. Elife 7:N/A (2018). Read more (PubMed: 29561262) »
- Tsantoulas C et al. Mice lacking Kcns1 in peripheral neurons show increased basal and neuropathic pain sensitivity. Pain 159:1641-1651 (2018). Read more (PubMed: 29697531) »