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    peripherin-antibody-ab53051.pdf

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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments
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Anti-Peripherin antibody (ab53051)

  • Datasheet
  • SDS
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Western blot - Anti-Peripherin antibody (ab53051)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody (ab53051)

Key features and details

  • Rabbit polyclonal to Peripherin
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Peripherin protein (ab114335)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Peripherin antibody
    See all Peripherin primary antibodies
  • Description

    Rabbit polyclonal to Peripherin
  • Host species

    Rabbit
  • Specificity

    ab53051 detects endogenous levels of total Peripherin protein.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    A synthetic peptide derived from human Peripherin.

  • Positive control

    • Human brain tissue and HepG2 cell extracts.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab53051 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Peripherin
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Hep G2 cytoplasmic extract lysate (ab14659)
  • Recombinant Protein

    • Recombinant Human Peripherin protein (ab114335)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
WB
1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P (1)
Use at an assay dependent concentration.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Class-III neuronal intermediate filament protein.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Target information above from: UniProt accession P41219 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5630 Human
    • Entrez Gene: 19132 Mouse
    • Omim: 170710 Human
    • SwissProt: P41219 Human
    • SwissProt: P15331 Mouse
    • Unigene: 37044 Human
    • Unigene: 2477 Mouse
    • Alternative names

      • NEF 4 antibody
      • NEF4 antibody
      • Neurofilament 4 (57kD) antibody
      • Neurofilament 4 antibody
      • Perf antibody
      • PERI_HUMAN antibody
      • Peripherin antibody
      • PRPH 1 antibody
      • prph antibody
      • PRPH1 antibody
      see all

    Images

    • Western blot - Anti-Peripherin antibody (ab53051)
      Western blot - Anti-Peripherin antibody (ab53051)
      All lanes : Anti-Peripherin antibody (ab53051) at 1/500 dilution

      Lane 1 : HepG2 cell extract
      Lane 2 : HepG2 cell extract with immunising peptide

      Predicted band size: 54 kDa
      Observed band size: 54 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody (ab53051)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody (ab53051)
      ab53051 at 1/50 dilution staining Peripherin in human brain by Immunohistochemistry, Paraffin embedded tissue (in the absence and presence of the immunising peptide).

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab53051? Please let us know so that we can cite the reference in this datasheet.

    ab53051 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peripherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung adenocarcinoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 8.5 buffer
    Permeabilization
    No
    Specification
    Lung adenocarcinoma
    Fixative
    Formaldehyde
    Read More

    Mr. Allan Wang

    Verified customer

    Submitted Mar 20 2018

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