- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Peripherin antibody
See all Peripherin primary antibodies -
DescriptionRabbit polyclonal to Peripherin
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Host speciesRabbit
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Specificityab53051 detects endogenous levels of total Peripherin protein.
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Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
A synthetic peptide derived from human Peripherin.
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Positive control
- Human brain tissue and HepG2 cell extracts.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab53051 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab53051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/5000. |
Target
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FunctionClass-III neuronal intermediate filament protein.
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Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
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Database links
- Entrez Gene: 5630 Human
- Entrez Gene: 19132 Mouse
- Omim: 170710 Human
- SwissProt: P41219 Human
- SwissProt: P15331 Mouse
- Unigene: 37044 Human
- Unigene: 2477 Mouse
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Alternative names
- NEF 4 antibody
- NEF4 antibody
- Neurofilament 4 (57kD) antibody
see all
Images
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Western blot - Anti-Peripherin antibody (ab53051)All lanes : Anti-Peripherin antibody (ab53051) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide
Predicted band size: 54 kDa
Observed band size: 54 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peripherin antibody (ab53051)ab53051 at 1/50 dilution staining Peripherin in human brain by Immunohistochemistry, Paraffin embedded tissue (in the absence and presence of the immunising peptide).
References
ab53051 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"