Key features and details
- Rabbit polyclonal to Periplakin
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Periplakin antibody
See all Periplakin primary antibodies
DescriptionRabbit polyclonal to Periplakin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Recombinant fragment (His-T7-tag) corresponding to Human Periplakin aa 1439-1716. N-terminal tags. Expressed in E.coli.
EKVTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLA ALEKAEVKEKVVLSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVS RLEARLSELEFHNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEINM AATETRDLRNMTVADSGTNHDSRLWSLERELDDLKRLSKDKDLEIDELQK RLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLIDWNMFVKL RSQECDWEEISVKGPNGESSVIHDRKSG
Database link: O60437
- IHC-P: Human brain tissue. WB: Recombinant human Periplakin protein; Pig skin lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionComponent of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
Tissue specificityExpressed in stratified squamous epithelia and in some other epithelia.
Sequence similaritiesBelongs to the plakin or cytolinker family.
Contains 2 plectin repeats.
Contains 4 spectrin repeats.
Cellular localizationCell junction > desmosome. Cytoplasm > cytoskeleton. Cell membrane. Nucleus. Mitochondrion. Associated with desmosomes and intermediate filaments.
- Information by UniProt
- 190 kDa paraneoplastic pemphigus antigen antibody
- 195 kDa cornified envelope precursor antibody
- 195 kDa cornified envelope precursor protein antibody
Formalin-fixed, paraffin-embedded human brain tissue stained for periplakin using ab233128 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Anti-Periplakin antibody (ab233128) at 5 µg/ml + Pig skin tissue lysate
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml
Anti-Periplakin antibody (ab233128) at 2 µg/ml + Recombinant human Periplakin protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233128 has not yet been referenced specifically in any publications.