Key features and details
- Rabbit polyclonal to Periplakin
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Periplakin antibody
See all Periplakin primary antibodies
DescriptionRabbit polyclonal to Periplakin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Guinea pig, Cow, Xenopus laevis, Chimpanzee, Amphibian, Rhesus monkey, Gorilla, Orangutan, Medaka fish
Synthetic peptide corresponding to a region between residues 1150 and 1200 of human Periplakin (NP_002696.3)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72422 was affinity purified using an epitope specific to Periplakin immobilized on solid support.
Our Abpromise guarantee covers the use of ab72422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComponent of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
Tissue specificityExpressed in stratified squamous epithelia and in some other epithelia.
Sequence similaritiesBelongs to the plakin or cytolinker family.
Contains 2 plectin repeats.
Contains 4 spectrin repeats.
Cellular localizationCell junction > desmosome. Cytoplasm > cytoskeleton. Cell membrane. Nucleus. Mitochondrion. Associated with desmosomes and intermediate filaments.
- Information by UniProt
- 190 kDa paraneoplastic pemphigus antigen antibody
- 195 kDa cornified envelope precursor antibody
- 195 kDa cornified envelope precursor protein antibody
All lanes : Anti-Periplakin antibody (ab72422) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 205 kDa
Observed band size: 205 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
Detection of Periplakin by Western Blot of Immunprecipitate.
ab72422 at 1µg/ml staining Periplakin in HeLa whole cell lysate immunoprecipitated using ab72422 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab72422 has been referenced in 1 publication.
- Goldie SJ et al. Loss of GRHL3 leads to TARC/CCL17-mediated keratinocyte proliferation in the epidermis. Cell Death Dis 9:1072 (2018). PubMed: 30341279