Anti-Periplakin antibody (ab72422)


  • Product name
  • Description
    Rabbit polyclonal to Periplakin
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Guinea pig, Cow, Xenopus laevis, Chimpanzee, Amphibian, Rhesus monkey, Gorilla, Orangutan, Medaka fish
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1150 and 1200 of human Periplakin (NP_002696.3)

  • Positive control
    • HeLa whole cell lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: sw480.



Our Abpromise guarantee covers the use of ab72422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function
    Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
  • Tissue specificity
    Expressed in stratified squamous epithelia and in some other epithelia.
  • Sequence similarities
    Belongs to the plakin or cytolinker family.
    Contains 2 plectin repeats.
    Contains 4 spectrin repeats.
  • Cellular localization
    Cell junction > desmosome. Cytoplasm > cytoskeleton. Cell membrane. Nucleus. Mitochondrion. Associated with desmosomes and intermediate filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • 190 kDa paraneoplastic pemphigus antigen antibody
    • 195 kDa cornified envelope precursor antibody
    • 195 kDa cornified envelope precursor protein antibody
    • AW553870 antibody
    • KIAA0568 antibody
    • MGC134872 antibody
    • PEPL_HUMAN antibody
    • Periplakin antibody
    • ppl antibody
    see all


  • ICC/IF image of ab72422 stained SW480 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72422 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-Periplakin antibody (ab72422) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 205 kDa
    Observed band size: 205 kDa
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 10 seconds
  • Detection of Periplakin by Western Blot of Immunprecipitate.
    ab72422 at 1µg/ml staining Periplakin in HeLa whole cell lysate immunoprecipitated using ab72422 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.


ab72422 has not yet been referenced specifically in any publications.

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