Key features and details
- Rabbit polyclonal to Periplakin
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Periplakin antibody
See all Periplakin primary antibodies
DescriptionRabbit polyclonal to Periplakin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Guinea pig, Cow, Xenopus laevis, Chimpanzee, Amphibian, Rhesus monkey, Gorilla, Orangutan, Medaka fish
Synthetic peptide corresponding to a region between residues 1150 and 1200 of human Periplakin (NP_002696.3)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72422 was affinity purified using an epitope specific to Periplakin immobilized on solid support.
Our Abpromise guarantee covers the use of ab72422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComponent of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
Tissue specificityExpressed in stratified squamous epithelia and in some other epithelia.
Sequence similaritiesBelongs to the plakin or cytolinker family.
Contains 2 plectin repeats.
Contains 4 spectrin repeats.
Cellular localizationCell junction > desmosome. Cytoplasm > cytoskeleton. Cell membrane. Nucleus. Mitochondrion. Associated with desmosomes and intermediate filaments.
- Information by UniProt
- 190 kDa paraneoplastic pemphigus antigen antibody
- 195 kDa cornified envelope precursor antibody
- 195 kDa cornified envelope precursor protein antibody
All lanes : Anti-Periplakin antibody (ab72422) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 205 kDa
Observed band size: 205 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
Detection of Periplakin by Western Blot of Immunprecipitate.
ab72422 at 1µg/ml staining Periplakin in HeLa whole cell lysate immunoprecipitated using ab72422 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab72422 has been referenced in 1 publication.
- Goldie SJ et al. Loss of GRHL3 leads to TARC/CCL17-mediated keratinocyte proliferation in the epidermis. Cell Death Dis 9:1072 (2018). PubMed: 30341279