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Our lab recent purchased a large batch of antibodies from your company. Purchase Order *****. Two of which are not working correctly.
I have attachted a word document containing Western blots using the two primary antibodies:-
Anti-Periplakin antibody - (ab72422)
Anti-KIF14 antibody - (ab3746)
These western blots have been exposed for 15 minutes. Whereas, the Abcam info sheets state 10 seconds required.
The Periplakin blot shows a band at approximately 55 kDa that appeared after 10 seconds, however the band just below 200 kDa (which I believe to be periplakin) wasn't picked up until 15 minutes exposure.
The KIF14 blots shows a band at just below 200kDa (which I believe to be KIF14), however this wasn't picked up until 15 minutues exposure.
The samples that I used to perform this experiment, are the same samples used to conduct MS/MS-SILAC and the results of this confirmed that there was a drastic increase in these proteins in these samples compared to mock whole cell lysate. Hence I should be seeing a strong band for both KIF14 and Periplakin.
Is there any alternativies or a differnt batch of these antibodies that you can send us?
I look forward to hearing from you.
Asked on Jul 03 2012
Thank you for contacting us and for providing this further information.
As explained over the phone,I appreciate the time you have spent on these experiments and would be pleased to arrange a replacement, a refund or credit note in compensation.
As replacement please find below the list of alternative productswe can send to you. Please note that these 3 antibodies are currently out of stock and should be delivered to you withina week.
anti-KIF14, ab3747, https://www.abcam.com/ab3747
anti-KIF14, ab71155, https://www.abcam.com/ab71155
anti-periplatin, ab131269, https://www.abcam.com/ab131269
I look forward to hearing from you with details of how you would like to proceed.
Answered on Jul 03 2012