Recombinant Anti-Periplakin antibody [EPR8296] (ab131269)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Periplakin antibody [EPR8296]
See all Periplakin primary antibodies -
Description
Rabbit monoclonal [EPR8296] to Periplakin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Periplakin aa 150-250. The exact sequence is proprietary.
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Positive control
- HACAT and Human skin lysates; Human skin tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.82 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8296 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab131269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 195 kDa (predicted molecular weight: 205 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
Target
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Function
Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling. -
Tissue specificity
Expressed in stratified squamous epithelia and in some other epithelia. -
Sequence similarities
Belongs to the plakin or cytolinker family.
Contains 2 plectin repeats.
Contains 4 spectrin repeats. -
Cellular localization
Cell junction > desmosome. Cytoplasm > cytoskeleton. Cell membrane. Nucleus. Mitochondrion. Associated with desmosomes and intermediate filaments. - Information by UniProt
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Database links
- Entrez Gene: 5493 Human
- Omim: 602871 Human
- SwissProt: O60437 Human
- Unigene: 192233 Human
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Alternative names
- 190 kDa paraneoplastic pemphigus antigen antibody
- 195 kDa cornified envelope precursor antibody
- 195 kDa cornified envelope precursor protein antibody
see all
Images
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![Western blot - Anti-Periplakin antibody [EPR8296] (ab131269)](https://www.abcam.com/ps/products/131/ab131269/Images/ab131269-145634-anti-periplakin-antibody-epr8296-western-blot.jpg)
Western blot - Anti-Periplakin antibody [EPR8296] (ab131269)All lanes : Anti-Periplakin antibody [EPR8296] (ab131269) at 1/1000 dilution
Lane 1 : HACAT lysate
Lane 2 : Human skin lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 205 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periplakin antibody [EPR8296] (ab131269)](https://www.abcam.com/ps/products/131/ab131269/Images/ab131269-145633-anti-periplakin-antibody-epr8296-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periplakin antibody [EPR8296] (ab131269)Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Periplakin with ab131269 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Other - Anti-Periplakin antibody [EPR8296] (ab131269)](https://www.abcam.com/ps/products/131/ab131269/Images/ab131269-145632-anti-periplakin-antibody-epr8296.jpg)
Other - Anti-Periplakin antibody [EPR8296] (ab131269)
Datasheets and documents
References
This product has been referenced in:
- Lee HM et al. The 4717C?>?G polymorphism in periplakin modulates sensitivity to EGFR inhibitors. Sci Rep 9:2357 (2019). Read more (PubMed: 30787334) »
