Product nameAnti-Periplakin antibody [EPR8296]
See all Periplakin primary antibodies
DescriptionRabbit monoclonal [EPR8296] to Periplakin
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Periplakin aa 150-250. The exact sequence is proprietary.
- HACAT and Human skin lysates; Human skin tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.82 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 195 kDa (predicted molecular weight: 205 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
FunctionComponent of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
Tissue specificityExpressed in stratified squamous epithelia and in some other epithelia.
Sequence similaritiesBelongs to the plakin or cytolinker family.
Contains 2 plectin repeats.
Contains 4 spectrin repeats.
Cellular localizationCell junction > desmosome. Cytoplasm > cytoskeleton. Cell membrane. Nucleus. Mitochondrion. Associated with desmosomes and intermediate filaments.
- Information by UniProt
- 190 kDa paraneoplastic pemphigus antigen antibody
- 195 kDa cornified envelope precursor antibody
- 195 kDa cornified envelope precursor protein antibody
All lanes : Anti-Periplakin antibody [EPR8296] (ab131269) at 1/1000 dilution
Lane 1 : HACAT lysate
Lane 2 : Human skin lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 205 kDa
Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Periplakin with ab131269 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
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