Product nameAnti-PERK antibody
See all PERK primary antibodies
DescriptionRabbit polyclonal to PERK
SpecificityThis antibody detects endogenous levels of total PERK protein.
Tested applicationsSuitable for: ELISA, ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
A synthesized peptide derived from an internal region of human PERK
- HepG2 cells and extracts from Hela and HepG2 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|WB||1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionPhosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
Tissue specificityUbiquitous. A high level expression is seen in secretory tissues.
Involvement in diseaseDefects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 1 protein kinase domain.
DomainThe lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- DKFZp781H1925 antibody
- E2AK3_HUMAN antibody
- EC 184.108.40.206 antibody
All lanes : Anti-PERK antibody (ab65142) at 1/500 dilution
Lane 1 : Extracts from Hela cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from HepG2 cells, plus immunising peptide
Predicted band size: 125 kDa
Observed band size: 125 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug.
Immunofluorescence analysis of HepG2 cells using ab65142 at a 1/500-1/1000 dilution. Left image untreated. Right image treated with immunising peptide.
PERK was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to PERK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65142.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .
Band: 130kDa: PERK.
This product has been referenced in:
- Kohn TP et al. Whole-Exome Sequencing Identifies Novel Heterozygous Mutation in RAF1 in Family With Neonatal Testicular Torsion. Urology N/A:N/A (2019). Read more (PubMed: 30904638) »
- Guo Y et al. Carnosine improves diabetic retinopathy via the MAPK/ERK pathway. Exp Ther Med 17:2641-2647 (2019). Read more (PubMed: 30930967) »