Anti-PERK antibody (ab65142)
Key features and details
- Rabbit polyclonal to PERK
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PERK antibody
See all PERK primary antibodies -
Description
Rabbit polyclonal to PERK -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthesized peptide derived from an internal region of human PERK
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
1/500 - 1/1000.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa).
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Notes |
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ICC/IF
1/500 - 1/1000. |
WB
1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa). |
Target
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Function
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. -
Tissue specificity
Ubiquitous. A high level expression is seen in secretory tissues. -
Involvement in disease
Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 1 protein kinase domain. -
Domain
The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP. -
Post-translational
modificationsAutophosphorylated.
N-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 9451 Human
- Omim: 604032 Human
- SwissProt: Q9NZJ5 Human
- Unigene: 591589 Human
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Alternative names
- DKFZp781H1925 antibody
- E2AK3_HUMAN antibody
- EC 2.7.11.1 antibody
see all
Images
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All lanes : Anti-PERK antibody (ab65142) at 1/500 dilution
Lane 1 : Extracts from Hela cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from HepG2 cells, plus immunising peptide
Predicted band size: 125 kDa
Observed band size: 125 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug. -
Immunofluorescence analysis of HepG2 cells using ab65142 at a 1/500-1/1000 dilution. Left image untreated. Right image treated with immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (61)
ab65142 has been referenced in 61 publications.
- Zhang WY et al. Long noncoding RNA Gas5 induces cell apoptosis and inhibits tumor growth via activating the CHOP-dependent endoplasmic reticulum stress pathway in human hepatoblastoma HepG2 cells. J Cell Biochem 123:231-247 (2022). PubMed: 34636091
- Shao X et al. Exploring the Mechanism of Weikang Keli in Inhibiting Gastric Cancer through the MAPK Signaling Pathway: Based on Network Pharmacology and Experimental Verification. Evid Based Complement Alternat Med 2022:2662288 (2022). PubMed: 35547655
- Saptarshi N et al. PERK/EIF2AK3 integrates endoplasmic reticulum stress-induced apoptosis, oxidative stress and autophagy responses in immortalised retinal pigment epithelial cells. Sci Rep 12:13324 (2022). PubMed: 35922637
- Liang Y et al. Inhibition of the IRE1/JNK pathway in renal tubular epithelial cells attenuates ferroptosis in acute kidney injury. Front Pharmacol 13:927641 (2022). PubMed: 36091771
- Liu X et al. Qingfei Tongluo Formula Mitigates Mycoplasma pneumoniae Infection via the PERK Signaling Pathway. Dis Markers 2022:9340353 (2022). PubMed: 36523813