Western blot abreview for Anti-PERK (phospho T982) antibody

Below Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-486 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycin gel)
Loading amount
50000 cells
Specification
MDA-MB-486 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/500

Secondary antibody

Dilution
1/10000
Name

Detection

Detection method
BioRad Clarity Western ECL
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 170 kDa Non-specific: 50, 60 kDa

Additional data

Abcam response
Thank you for having tested this antibody in an untested application ICC/IF. For your information, we can offer AbTrial discount code to test our products in an untested application or species without financial risk. https://www.abcam.com/content/abtrial-program-test-risk-free The intensity of the specific band can be optimised by stimulating cells with thapsigargin (Tg) as described in this Abreview: https://www.abcam.com/PERK-phospho-T982-antibody-ab192591/reviews/55027

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Submitted Dec 07 2016

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