Western blot abreview for Anti-PERK (phospho T982) antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
50 µg
Treatment
Tunicamycin 10 ´M for 1h, 3h, 24h
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA, TBS, tween 0.1%
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Cell Signaling Anti-rabbit IgG, HRP-linked Antibod
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
Lysate from untreated HeLa cells
Exposure
30 second(s)
Bands
Specific: 180 kDa
Positive control
Lysate from HeLa cells treated with 10 ´M tunicamycin for 1h, 3h, 24h

Additional data

Additional Notes
Lane 1: ctrl Lanes 2-5: our experimental treatment for 30 min, 1h, 2h, 3h Lane 6: 10 ´M tunicamycin for 1h Lane 7: 10 ´M tunicamycin for 3h Lane 8: 10 ´M tunicamycin for 24h. Lysis buffer: 1x RIPA (ab156034) supplemented with Protease and Phosphatase Inhibitor Cocktail (ab201119).

Dr. Tamas Czompoly

Verified customer

Submitted May 19 2017

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