Western blot abreview for Anti-PERK (phospho T982) antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (mutiple myeloma)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
100 ng/ml LPS
Specification
mutiple myeloma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti-rabbit secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Exposure
30 second(s)
Bands
Specific: 42, 44 kDa

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Submitted Jun 26 2018

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