Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PERK (phospho T982) antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Skin
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/1000

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: Goat anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
This antibody, in my hands, should be diluted further. Intense positivity is seen in the cytoplasm of developing basal skin cells. There is also a distinct positivity in the cytoplasm of developing skeletal muscle fibres (and in heart and smooth muscle not shown). Mouse skin and human mucosa (tonsil) give an identical basal cell positivity. Results are similar (I think better) than those obtained by HPA on human tissue (enhanced orthogonal abs).

Mr. Carl Hobbs

Verified customer

Submitted Jul 26 2019

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