Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PERK (phospho T982) antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/2000

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: Goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Submitted image shows mucosa of tonsil. The positivity of the basal cells of the mucosa is intense: the primary antibody should be diluted further thus removing the moderate? non-specific? positivity of the epithelial upper layers. Using this antibody on mouse and rat sections (under identical conditions) the basal layer of developing skin epithelium is positive. Results are similar (I think better) than those obtained by HPA (enhanced orthogonal abs).

Mr. Carl Hobbs

Verified customer

Submitted Jul 26 2019

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