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    peroxidase-activity-assay-kit-ab155895.pdf

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Peroxidase Activity Assay Kit (ab155895)

  • Datasheet
  • SDS
  • Protocol Booklet
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H2O2 standard curve - fluorometric
  • H2O2 Standard Curve - Colorimetric

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric/Fluorometric
  • Platform: Microplate reader
  • Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine

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Overview

  • Product name

    Peroxidase Activity Assay Kit
    See all HRP kits
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • Assay type

    Enzyme activity
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Abcam’s Peroxidase Assay Kit (ab155895) provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the redfluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (λmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.

  • Notes

    Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    HRP Positive Control Green 1 vial
    Hydrogen Peroxide Substrate Yellow 1 x 0.1ml
    OxiRed Probe Red 1 x 0.2ml
    Peroxidase Assay Buffer WM 1 x 25ml
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Oxidative Stress Assay Kits
    • Oxidative Stress
  • Relevance

    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
  • Cellular localization

    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
  • Alternative names

    • Horseradish Peroxidase
    • HPRC1
    • HRP
    • Peroxidase
    • Peroxidase C1A
    • PRXC1A
    see all

Associated products

    Images

    • H2O2 standard curve - fluorometric
      H2O2 standard curve - fluorometric
      An example of a Fluorimetric Standard Curve
    • H2O2 Standard Curve - Colorimetric
      H2O2 Standard Curve - Colorimetric
      An example of a Colorimetric Standard Curve

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab155895? Please let us know so that we can cite the reference in this datasheet.

    ab155895 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    For reaction mix, do I use the same amount of OxiRed Probe solution for both colorimetric and fluometric test?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 06 2013

    Answer

    Yes, for this assay protocol you need to use the same amount of probe irrespective of your analysis mode.

    Read More

    Abcam Scientific Support

    Answered on Jun 06 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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