Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
Product namePeroxidase Activity Assay Kit
See all HRP kits
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids
Assay typeEnzyme activity
Species reactivityReacts with: Mammals, Other species
Abcam’s Peroxidase Assay Kit (ab155895) provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the redfluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (λmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests HRP Positive Control Green 1 vial Hydrogen Peroxide Substrate Yellow 1 x 0.1ml OxiRed Probe Red 1 x 0.2ml Peroxidase Assay Buffer WM 1 x 25ml
RelevanceHorseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Cellular localizationSecreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
- Horseradish Peroxidase
ab155895 has not yet been referenced specifically in any publications.