Peroxidase Activity Assay Kit (ab155895)

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Overview

  • Product name
    Peroxidase Activity Assay Kit
  • Detection method
    Colorimetric/Fluorometric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • Assay type
    Enzyme activity
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Abcam’s Peroxidase Assay Kit (ab155895) provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the redfluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (λmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.

  • Notes

    Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.

  • Platform
    Microplate reader

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    HRP Positive Control Green 1 vial
    Hydrogen Peroxide Substrate Yellow 1 x 0.1ml
    OxiRed Probe Red 1 x 0.2ml
    Peroxidase Assay Buffer WM 1 x 25ml
  • Research areas
  • Relevance
    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
  • Cellular localization
    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
  • Alternative names
    • Horseradish Peroxidase
    • HPRC1
    • HRP
    • Peroxidase
    • Peroxidase C1A
    • PRXC1A
    see all

Images

  • H2O2 standard curve - fluorometric
    H2O2 standard curve - fluorometric
    An example of a Fluorimetric Standard Curve
  • H2O2 Standard Curve - Colorimetric
    H2O2 Standard Curve - Colorimetric
    An example of a Colorimetric Standard Curve

Protocols

References

ab155895 has not yet been referenced specifically in any publications.

Publishing research using ab155895? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

For reaction mix, do I use the same amount of OxiRed Probe solution for both colorimetric and fluometric test?

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Answer

Yes, for this assay protocol you need to use the same amount of probe irrespective of your analysis mode.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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