Product namePeroxide Assay Kit
See all Hydrogen Peroxide kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media
Range0.2 µM - 30 µM
Peroxide Assay Kit (ab272537) is a simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. This assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe3+-xylenol orange reaction, in which a purple complex is formed when Fe2+ provided in the reagent is oxidized to Fe3+ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 µM (7 ng/mL) to 30 µM (1,020 ng/mL) H2O2 in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 250 tests Reagent A 1 x 1ml Reagent B 1 x 50ml Standard (3% stabilized H2O2) 1 x 100µl
RelevanceHydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases.
Our Abpromise guarantee covers the use of ab272537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab272537 has not yet been referenced specifically in any publications.