Anti-Peroxiredoxin 1/PAG antibody (ab109766)
Key features and details
- Goat polyclonal to Peroxiredoxin 1/PAG
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Peroxiredoxin 1/PAG antibody
See all Peroxiredoxin 1/PAG primary antibodies -
Description
Goat polyclonal to Peroxiredoxin 1/PAG -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human Peroxiredoxin 1/PAG aa 100-200 (internal sequence).
Database link: NP_859048.1 -
Positive control
- IHC-P: placenta or thyroid tissue. WB: HepG2, HEK293 or A431 cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 23-24 kDa (predicted molecular weight: 22 kDa).
|
|
IHC-P |
Use a concentration of 2.5 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 23-24 kDa (predicted molecular weight: 22 kDa). |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
-
Function
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. -
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Post-translational
modificationsPhosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Database links
- Entrez Gene: 281997 Cow
- Entrez Gene: 5052 Human
- Entrez Gene: 18477 Mouse
- Entrez Gene: 117254 Rat
- Omim: 176763 Human
- SwissProt: Q9JKY1 Chinese hamster
- SwissProt: Q5E947 Cow
- SwissProt: Q06830 Human
see all -
Alternative names
- Heme binding 23 kDa protein antibody
- MSP23 antibody
- Natural killer cell-enhancing factor A antibody
see all
Images
-
Anti-Peroxiredoxin 1/PAG antibody (ab109766) at 0.01 µg/ml (incubation time: 1 hour) + HepG2 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 22 kDa -
ab109766 at 2.5µg/ml staining Peroxiredoxin 1/PAG in formalin-fixed, paraffin-embedded Human placenta tissue.
-
ab109766 at 2.5µg/ml staining Peroxiredoxin 1/PAG in formalin-fixed, paraffin-embedded Human thyroid tissue.
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab109766 has not yet been referenced specifically in any publications.