• Product name
    Anti-Peroxiredoxin 1/PAG antibody [13E7]
    See all Peroxiredoxin 1/PAG primary antibodies
  • Description
    Mouse monoclonal [13E7] to Peroxiredoxin 1/PAG
  • Host species
  • Tested applications
    Suitable for: IP, ICC/IF, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Peroxiredoxin 1/PAG.

  • Positive control
    • HeLa cell lysate
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.



Our Abpromise guarantee covers the use of ab16745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 1 - 2 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.1-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
  • Sequence similarities
    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Post-translational
    Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heme binding 23 kDa protein antibody
    • MSP23 antibody
    • Natural killer cell-enhancing factor A antibody
    • NKEF A antibody
    • NKEF-A antibody
    • NKEFA antibody
    • OSF3 antibody
    • Osteoblast specific factor 3 antibody
    • PAG antibody
    • Paga antibody
    • PAGB antibody
    • Peroxiredoxin 1 antibody
    • Peroxiredoxin-1 antibody
    • PRDX1 antibody
    • PRDX1_HUMAN antibody
    • Proliferation associated gene A antibody
    • Proliferation-associated gene protein antibody
    • PRX1 antibody
    • PrxI antibody
    • TDPX2 antibody
    • Thioredoxin peroxidase 2 antibody
    • Thioredoxin-dependent peroxide reductase 2 antibody
    see all


  • Overlay histogram showing HeLa cells stained with ab16745 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16745, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ICC/IF image of ab16745 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16745, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab16745 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


1) Abcam product code ab16745

2) Abcam order reference number or product batch number Lot #GR78614

3) Description of the problem When used for IP, not pulling down as much higher molecular weight interacting molecules, although Prx1 dimer (˜37kDa) is pulled down efficiently.

4) Sample preparation:

Type of sample (whole cell lysates, fraction, recombinant protein…): Whole cell lysates

Lysis buffer

Protease inhibitors: Roche mini protease inhibitor (EDTA free)

Phosphatase inhibitors

Amount of Lysate IPed ug or number of cells 200µg

Lysate precleared with matrix : yes/no No

Positive control Untreated cells with antibody

Negative control Untreated cells without antibody

5) IP antibody: mouse monoclonal against Prx1

Amount/concentration or dilution used : 12-24µg/mL


Antibody noncovalently bound to matrix before incubation with lysate sample?

Antibody noncovalently bound to matrix after incubation with lysate sample?

Antibody covalently bound to matrix - please describe briefly?

Antibody-lysate incubation time: 9 hours

Incubation temperature : 4 degrees Celcius

6) IP Matrix (e. beads): Protein G agarose

Type of matrix

Amount of matrix

7) Washing after antibody-lysate incubation:

Buffer: Cell lysis buffer

Number of washes : 3-6

8) IP analysis:

Verification method: e.g reprobe in western blotting Western blot

Describe verification method briefly: After IP has been eluted with SDS loading buffer (˜25µL, loaded immediately onto 7.5% SDS gel)

OR if Western blotting reprobe performed please indicate the following:

Reducing agent

Boiling for ≥5 min? Yes

Protein loaded ug/lane or cells/lane Not sure

Percentage of gel 7.5%

Type of membrane PVDF

Protein transfer verified Post stain gel with Coomasie

Blocking agent and concentration SeaBlock, 1 in 10 dilution from manufacturer stock

Blocking time 1 hour

Blocking temperature Room temperature

Primary antibody (If more than one was used, describe in “additional notes”): Rabbit polyclonal to Prx1 (ab41906)

Concentration or dilution 1:5000

Diluent buffer TBS, with 0.1% Tween

Incubation time Overnight

Incubation temperature: 4 degrees Celcius

Secondary antibody: Goat anti rabbit, HRP conjugated


Reacts against:

Concentration or dilution 1:50,000

Diluent buffer TBS with 0.1% Tween

Incubation time 1 hour

Incubation temperature: Room temperature

Fluorochrome or enzyme conjugate:

Washing after primary and secondary antibodies:

Buffer TBS with 0.1% Tween

Number of washes 10 mins X 2 or 5 mins X 4

Detection method ECL

Was the method successfully used to detect protein from non-IPed samples? Yes

Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem

Read More

Thank you for filling in the questionnaire.

I am sorry to hear that ab16745 is not resulting in optimal results, although Prx1 is pulled down.

Firstly, to the immunogen and epitope sequences: The lab confirmed that for ab16745 (as for ab16746 and ab16747) the full-length sequence of Prx1 produced in E.coli was used. Unfortunately, the epitope has not been mapped. It could indeed be possible that the antibody binding site is masked by your protein of interest.

Your protocol looks alright (maybe with exception of the very high concentration of antibody used, as 1-2 ug/ml should be sufficient),so I am not quite sure if thereare any suggestions that would help to improve the results. Maybe playing around with the lysis buffer might be an option to see if the complex is intact and can be pulled down, if you have not tried already. I have attached our IP protocol for further details, which can also be found on our website: https://www.abcam.com/index.html?pageconfig=popular_protocols.

Having said this however, this might depend also on the interacting proteins you are investigating. It sounds as if you know this protein/these proteins. Therefore, I would suggest to try the IP with an antibodyfor the interacting protein if possible; if it works, this would indicate that the IP conditions are ok and that the epitope for ab16745 is indeed masked.

If you are looking for an alternative anti-Peroxiredoxin 1 antibody, I am happy to let you know that we have (apart from the ones mentioned above) two more available that are suitable for IP of human Peroxiredoxin 1:

ab41906 (which you already use for the WB verification): Theimmunogen is x (amino acids y-z), and thus might be an alternative worth testing (depending on the binding site of your protein(s) of interest).

ab109506 wasgenerated against a synthetic peptide corresponding to residues near the C-terminus of Human Peroxiredoxin 1, and I can find out the details for you if your are interested.

https://www.abcam.com/index.html?datasheet=109506 (or use the following: https://www.abcam.com/index.html?datasheet=109506).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (Hela)
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Xiao Qing Lu

Verified customer

Submitted Apr 03 2009


I'm sorry to hear you are having a problem with ab16745. To our knowledge, this antibody has yet to be tested in this application. Still, I would like to suggest the following modifications to your protocol to try and get this to work: 1. Try switching from milk to BSA. In addition, you may wish to remove the blocking agent from your primary solution. It is possible this is causing the blot to be blocked too much. 2. Try diluting the antibody 1:250. Please let me know if this helps and do not hesitate to contact us for further advice.

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