Overview

  • Product name

    Anti-Peroxiredoxin 1/PAG antibody
    See all Peroxiredoxin 1/PAG primary antibodies
  • Description

    Rabbit polyclonal to Peroxiredoxin 1/PAG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Peroxiredoxin 1/PAG (internal sequence). The exact sequence is proprietary.
    Database link: Q06830

  • Positive control

    • ICC/IF: HeLa cells. WB: HEK-293T and NIH/3T3 whole cell lysates. IHC-P: Mouse lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
  • Sequence similarities

    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Post-translational
    modifications

    Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Heme binding 23 kDa protein antibody
    • MSP23 antibody
    • Natural killer cell-enhancing factor A antibody
    • NKEF A antibody
    • NKEF-A antibody
    • NKEFA antibody
    • OSF3 antibody
    • Osteoblast specific factor 3 antibody
    • PAG antibody
    • Paga antibody
    • PAGB antibody
    • Peroxiredoxin 1 antibody
    • Peroxiredoxin-1 antibody
    • PRDX1 antibody
    • PRDX1_HUMAN antibody
    • Proliferation associated gene A antibody
    • Proliferation-associated gene protein antibody
    • PRX1 antibody
    • PrxI antibody
    • TDPX2 antibody
    • Thioredoxin peroxidase 2 antibody
    • Thioredoxin-dependent peroxide reductase 2 antibody
    see all

Images

  • Anti-Peroxiredoxin 1/PAG antibody (ab227122) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Predicted band size: 22 kDa



    12% SDS-PAGE gel.

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Peroxiredoxin 1/PAG (green) using ab227122 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).

  • Anti-Peroxiredoxin 1/PAG antibody (ab227122) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 22 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded mouse lung tissue stained for Peroxiredoxin 1/PAG using ab227122 at 1/500 dilution in immunohistochemical analysis.

References

ab227122 has not yet been referenced specifically in any publications.

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