Overview

  • Product name

    Anti-Peroxiredoxin 1/PAG antibody
    See all Peroxiredoxin 1/PAG primary antibodies
  • Description

    Rabbit polyclonal to Peroxiredoxin 1/PAG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Peroxiredoxin 1/PAG (internal sequence). The exact sequence is proprietary.
    Database link: Q06830

  • Positive control

    • WB: Mouse brain lysate; NCI-H1299, HeLa and HepG2 whole cell lysate (ab7900). IHC-P: HT-29 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
  • Sequence similarities

    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Post-translational
    modifications

    Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Heme binding 23 kDa protein antibody
    • MSP23 antibody
    • Natural killer cell-enhancing factor A antibody
    • NKEF A antibody
    • NKEF-A antibody
    • NKEFA antibody
    • OSF3 antibody
    • Osteoblast specific factor 3 antibody
    • PAG antibody
    • Paga antibody
    • PAGB antibody
    • Peroxiredoxin 1 antibody
    • Peroxiredoxin-1 antibody
    • PRDX1 antibody
    • PRDX1_HUMAN antibody
    • Proliferation associated gene A antibody
    • Proliferation-associated gene protein antibody
    • PRX1 antibody
    • PrxI antibody
    • TDPX2 antibody
    • Thioredoxin peroxidase 2 antibody
    • Thioredoxin-dependent peroxide reductase 2 antibody
    see all

Images

  • Paraffin-embedded HT-29 (human colorectal adenocarcinoma cell line) xenograft tissue stained for Peroxiredoxin 1/PAG using ab228780 at 1/100 dilution in immunohistochemical analysis.

  • Anti-Peroxiredoxin 1/PAG antibody (ab228780) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 22 kDa



    12% SDS-PAGE gel.

  • All lanes : Anti-Peroxiredoxin 1/PAG antibody (ab228780) at 1/1000 dilution

    Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 22 kDa



    12 % SDS-PAGE gel.

References

ab228780 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228780.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up