Anti-Peroxiredoxin 1/PAG antibody (ab41906)
- Datasheet
- References (18)
- Protocols
Overview
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Product nameAnti-Peroxiredoxin 1/PAG antibody
See all Peroxiredoxin 1/PAG primary antibodies -
DescriptionRabbit polyclonal to Peroxiredoxin 1/PAG
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, IP, WBmore details
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Species reactivityReacts with: Mouse, Rabbit, Human
Predicted to work with: Rat, Cow
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Immunogen
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Positive control
- WB: Recombinant human Peroxiredoxin 1/PAG protein (ab74172), HeLa, A431, Jurkat, HEK293, HepG2, MCF7, SHSY-5Y and U20S whole cell lysates. ICC/IF: HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab41906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 20631257 | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa). |
Target
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FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
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Sequence similaritiesBelongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Post-translational
modificationsPhosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. -
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 281997 Cow
- Entrez Gene: 5052 Human
- Entrez Gene: 18477 Mouse
- Entrez Gene: 117254 Rat
- Omim: 176763 Human
- SwissProt: Q5E947 Cow
- SwissProt: Q06830 Human
- SwissProt: P35700 Mouse
see all -
Alternative names
- Heme binding 23 kDa protein antibody
- MSP23 antibody
- Natural killer cell-enhancing factor A antibody
see all
Images
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Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab41906 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab41906 was shown to recognize Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used, along with additional cross-reactive bands. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab41906 and ab8245 (loading control to GAPDH) at a concentration of 1 µg/ml and diluted to 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)All lanes : Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody (ab41906)IHC image of Peroxiredoxin 1/PAG staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41906, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)This protein is a homodimer consisting of two subunits with an expected molecular weight of 23kDa. -
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody (ab41906)ICC/IF image of ab41906 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41906, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Morita K et al. Peroxiredoxin as a functional endogenous antioxidant enzyme in pronuclei of mouse zygotes. J Reprod Dev 64:161-171 (2018). Read more (PubMed: 29503398) »
- Zhang H et al. Differential expression of peroxiredoxin 3 in laryngeal squamous cell carcinoma. Oncotarget 8:3471-3480 (2017). WB . Read more (PubMed: 27966448) »
