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    peroxiredoxin-1pag-antibody-ab41906.pdf

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Validated using a knockout cell line

Anti-Peroxiredoxin 1/PAG antibody (ab41906)

  • Datasheet
Reviews (5)Q&A (1)References (35)

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Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
  • Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
  • Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
  • Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)

Key features and details

  • Rabbit polyclonal to Peroxiredoxin 1/PAG
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Peroxiredoxin 1/PAG antibody
    See all Peroxiredoxin 1/PAG primary antibodies
  • Description

    Rabbit polyclonal to Peroxiredoxin 1/PAG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Peroxiredoxin 1/PAG aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab41919, ab41920)

  • Positive control

    • WB: U-2 OS, HAP1, A-431 and Jurkat cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant human Peroxiredoxin 1/PAG protein (ab74172)
  • Recombinant Protein

    • Recombinant mouse Peroxiredoxin 1/PAG protein (Active) (ab219223)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab41906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (3)
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).

Target

  • Function

    Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
  • Sequence similarities

    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Post-translational
    modifications

    Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession Q06830 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281997 Cow
    • Entrez Gene: 5052 Human
    • Entrez Gene: 18477 Mouse
    • Entrez Gene: 117254 Rat
    • Omim: 176763 Human
    • SwissProt: Q5E947 Cow
    • SwissProt: Q06830 Human
    • SwissProt: P35700 Mouse
    • SwissProt: Q63716 Rat
    • Unigene: 180909 Human
    • Unigene: 30929 Mouse
    • Unigene: 2845 Rat
    see all
  • Alternative names

    • Heme binding 23 kDa protein antibody
    • MSP23 antibody
    • Natural killer cell-enhancing factor A antibody
    • NKEF A antibody
    • NKEF-A antibody
    • NKEFA antibody
    • OSF3 antibody
    • Osteoblast specific factor 3 antibody
    • PAG antibody
    • Paga antibody
    • PAGB antibody
    • Peroxiredoxin 1 antibody
    • Peroxiredoxin-1 antibody
    • PRDX1 antibody
    • PRDX1_HUMAN antibody
    • Proliferation associated gene A antibody
    • Proliferation-associated gene protein antibody
    • PRX1 antibody
    • PrxI antibody
    • TDPX2 antibody
    • Thioredoxin peroxidase 2 antibody
    • Thioredoxin-dependent peroxide reductase 2 antibody
    see all

Images

  • Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
    Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 µg)
    Lane 3: A431 cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab41906 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab41906 was shown to recognize Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used, along with additional cross-reactive bands. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab41906 and ab8245 (loading control to GAPDH) at a concentration of 1 µg/ml  and diluted to  1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody (ab41906)

    IHC image of Peroxiredoxin 1/PAG staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41906, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
    Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
    All lanes : Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1/1000 dilution

    Lane 1 : Wild-type U-2 OS cell lysate
    Lane 2 : PRDX1 knockout U-2 OS cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 22 kDa



    ab41906 was shown to react with PRDX1 in wild-type U-2 OS cells in Western blot with loss of signal observed in a PRDX1 knockout cell line. Wild-type U-2 OS and PRDX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab41906 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. This data was kindly provided by the YCharOS Inc., an open science company with the mission of characterizing every commercially available antibody reagent. Abcam are working with YCharOS to support their mission of antibody characterisation using knockout cell lines.

  • Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
    Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
    All lanes : Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
    Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
    Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1 µg/ml + Recombinant human Peroxiredoxin 1/PAG protein (ab79945) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa


    Exposure time: 30 seconds


    This protein is a homodimer consisting of two subunits with an expected molecular weight of 23kDa.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (35)

Publishing research using ab41906? Please let us know so that we can cite the reference in this datasheet.

ab41906 has been referenced in 35 publications.

  • Oka SI  et al. ß-Hydroxybutyrate, a Ketone Body, Potentiates the Antioxidant Defense via Thioredoxin 1 Upregulation in Cardiomyocytes. Antioxidants (Basel) 10:N/A (2021). PubMed: 34356388
  • Lovatt M  et al. Peroxiredoxin-1 regulates lipid peroxidation in corneal endothelial cells. Redox Biol 30:101417 (2020). PubMed: 31901729
  • Cásedas G  et al. The Metabolite Urolithin-A Ameliorates Oxidative Stress in Neuro-2a Cells, Becoming a Potential Neuroprotective Agent. Antioxidants (Basel) 9:N/A (2020). PubMed: 32098107
  • Cui S  et al. CD1d1 intrinsic signaling in macrophages controls NLRP3 inflammasome expression during inflammation. Sci Adv 6:N/A (2020). PubMed: 33087357
  • Stretton C  et al. 2-Cys peroxiredoxin oxidation in response to hydrogen peroxide and contractile activity in skeletal muscle: A novel insight into exercise-induced redox signalling? Free Radic Biol Med 160:199-207 (2020). PubMed: 32784030
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-Peroxiredoxin 1/PAG antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Aorta)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
50 µg
Treatment
Calcification Diet + Vitamin Ð3
Specification
Aorta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. D Richard

Verified customer

Submitted Jul 27 2015

Western blot abreview for Anti-Peroxiredoxin 1/PAG antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hippocampus neuronal cells HpL3-4)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
20 µg
Specification
Hippocampus neuronal cells HpL3-4
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 28 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-Peroxiredoxin 1 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted Nov 16 2009

Immunoprecipitation abreview for Anti-Peroxiredoxin 1/PAG antibody

Excellent
Abreviews
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (MDA231)
Total protein in input
500 µg
Immuno-precipitation step
Protein G
Specification
MDA231
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Xumei Wang

Verified customer

Submitted Aug 28 2008

Western blot abreview for Anti-Peroxiredoxin 1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Loading amount
30 µg
Specification
heart
Gel Running Conditions
Non-reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 09 2008

Question

no signal with ab15571 lot 1185612
milkor BSA blocking tried
O/N 4ºC
dilution 1/1000
Leucemia cell lines (Human and Mouse)

Read More

Abcam community

Verified customer

Asked on Mar 16 2012

Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab15571 que vous avez reçu ne fonctionne pas comme attendu.
Le numéro de commande de remplacement gratuitavec leproduit ab41906 est *****. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

N'hésitez pas à nous contacter lors d'une prochaine occasion.

Read More

Abcam Scientific Support

Answered on Mar 16 2012

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