Recombinant Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)
![Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)](https://www.abcam.com/ps/products/247/ab247879/Images/ab247879-395405-anti-peroxiredoxin-1pag-antibody-epr5433-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5433] to Peroxiredoxin 1/PAG - BSA and Azide free
- Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free
See all Peroxiredoxin 1/PAG primary antibodies -
Description
Rabbit monoclonal [EPR5433] to Peroxiredoxin 1/PAG - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T and Jurkat cell lysates.
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General notes
ab247879 is the carrier-free version of ab109498 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247879 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5433 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
|
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| Flow Cyt |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
|
Flow Cyt
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval.
|
Target
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Function
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. -
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Post-translational
modificationsPhosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 5052 Human
- Entrez Gene: 18477 Mouse
- Omim: 176763 Human
- SwissProt: Q06830 Human
- SwissProt: P35700 Mouse
- Unigene: 180909 Human
- Unigene: 30929 Mouse
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Alternative names
- Heme binding 23 kDa protein antibody
- MSP23 antibody
- Natural killer cell-enhancing factor A antibody
see all
Images
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Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)All lanes : Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : PRDX1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab109498).
Lanes 1-3: Merged signal (red and green). Green - ab109498 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.
ab109498 Anti-Peroxiredoxin 1/PAG antibody [EPR5433] was shown to specifically react with Peroxiredoxin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266842 (knockout cell lysate ab257040) was used. Wild-type and Peroxiredoxin knockout samples were subjected to SDS-PAGE. ab109498 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)](https://www.abcam.com/ps/products/247/ab247879/Images/ab247879-2-benefits-of-recombinant-antibodies.png)
Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247879 has not yet been referenced specifically in any publications.
