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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)

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  • Certificate of Compliance
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Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)
  • Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5433] to Peroxiredoxin 1/PAG - BSA and Azide free
  • Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

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Recombinant mouse Peroxiredoxin 1/PAG protein (Active) (ab219223)

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Overview

  • Product name

    Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free
    See all Peroxiredoxin 1/PAG primary antibodies
  • Description

    Rabbit monoclonal [EPR5433] to Peroxiredoxin 1/PAG - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T and Jurkat cell lysates.
  • General notes

    ab247879 is the carrier-free version of ab109498 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247879 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5433
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • KO cell lines

    • Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (ab266842)
  • KO cell lysates

    • Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell lysate (ab257040)
  • Recombinant Protein

    • Recombinant mouse Peroxiredoxin 1/PAG protein (Active) (ab219223)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval.

 

Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
    • Sequence similarities

      Belongs to the ahpC/TSA family.
      Contains 1 thioredoxin domain.
    • Post-translational
      modifications

      Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
    • Cellular localization

      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession Q06830 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5052 Human
      • Entrez Gene: 18477 Mouse
      • Omim: 176763 Human
      • SwissProt: Q06830 Human
      • SwissProt: P35700 Mouse
      • Unigene: 180909 Human
      • Unigene: 30929 Mouse
      • Alternative names

        • Heme binding 23 kDa protein antibody
        • MSP23 antibody
        • Natural killer cell-enhancing factor A antibody
        • NKEF A antibody
        • NKEF-A antibody
        • NKEFA antibody
        • OSF3 antibody
        • Osteoblast specific factor 3 antibody
        • PAG antibody
        • Paga antibody
        • PAGB antibody
        • Peroxiredoxin 1 antibody
        • Peroxiredoxin-1 antibody
        • PRDX1 antibody
        • PRDX1_HUMAN antibody
        • Proliferation associated gene A antibody
        • Proliferation-associated gene protein antibody
        • PRX1 antibody
        • PrxI antibody
        • TDPX2 antibody
        • Thioredoxin peroxidase 2 antibody
        • Thioredoxin-dependent peroxide reductase 2 antibody
        see all

      Images

      • Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)
        Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)
        All lanes : Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498) at 1/1000 dilution

        Lane 1 : Wild-type HEK293T cell lysate
        Lane 2 : PRDX1 knockout HEK293T cell lysate
        Lane 3 : Jurkat cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

        Predicted band size: 22 kDa
        Observed band size: 26 kDa
        why is the actual band size different from the predicted?



        This data was developed using the same antibody clone in a different buffer formulation (ab109498).

        Lanes 1-3: Merged signal (red and green). Green - ab109498 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.

         ab109498 Anti-Peroxiredoxin 1/PAG antibody [EPR5433] was shown to specifically react with Peroxiredoxin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266842 (knockout cell lysate ab257040) was used. Wild-type and Peroxiredoxin knockout samples were subjected to SDS-PAGE. ab109498 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)
        Anti-Peroxiredoxin 1/PAG antibody [EPR5433] - BSA and Azide free (ab247879)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab247879? Please let us know so that we can cite the reference in this datasheet.

      ab247879 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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