Product nameAnti-Peroxiredoxin 2/PRP antibody [1E8]
See all Peroxiredoxin 2/PRP primary antibodies
DescriptionMouse monoclonal [1E8] to Peroxiredoxin 2/PRP
Tested applicationsSuitable for: ELISA, WB, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Peroxiredoxin 2/PRP.
- HeLa cell lysate
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, HEPES, 0.01% BSA
Concentration information loading...
Purification notesPurified from ascites.
Light chain typekappa
Our Abpromise guarantee covers the use of ab50862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000 - 1/4000. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 200 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
Sequence similaritiesBelongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
- Information by UniProt
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Peroxiredoxin 2/PRP knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: LnCaP cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab50862 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab50862 was shown to specifically react with Peroxiredoxin 2/PRP when Peroxiredoxin 2/PRP knockout samples were used. Wild-type and Peroxiredoxin 2 knockout samples were subjected to SDS-PAGE. ab50862 and ab181602 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG (H + L) (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-Peroxiredoxin 2/PRP antibody [1E8] (ab50862) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HepG2 cell lysate
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Overlay histogram showing HeLa cells stained with ab50862 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56802, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Nowotny K et al. Oxidants produced by methylglyoxal-modified collagen trigger ER stress and apoptosis in skin fibroblasts. Free Radic Biol Med 120:102-113 (2018). WB ; Human . Read more (PubMed: 29550330) »
- Hinchy EC et al. Mitochondria-derived ROS activate AMP-activated protein kinase (AMPK) indirectly. J Biol Chem 293:17208-17217 (2018). Read more (PubMed: 30232152) »