Key features and details
- Rabbit polyclonal to Peroxiredoxin 2/PRP
- Suitable for: WB, IHC-Fr, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Peroxiredoxin 2/PRP antibody
See all Peroxiredoxin 2/PRP primary antibodies
DescriptionRabbit polyclonal to Peroxiredoxin 2/PRP
Tested applicationsSuitable for: WB, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Cow, Pig, Rhesus monkey
Recombinant fragment within Human Peroxiredoxin 2/PRP (internal sequence). The exact sequence is proprietary.
Database link: P32119
- WB: A549 and HCT 116 whole cell extracts. IHC-P: Human breast cancer tissue. IHC-Fr: Rat liver tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 16,22 kDa.|
|IHC-Fr||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
Sequence similaritiesBelongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
- Information by UniProt
- Epididymis secretory sperm binding protein Li 2a antibody
- HEL S 2a antibody
- MGC4104 antibody
All lanes : Anti-Peroxiredoxin 2/PRP antibody (ab226922) at 1/5000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 16,22 kDa
15% SDS-PAGE gel.
Frozen section of rat liver tissue stained for Peroxiredoxin 2/PRP using ab226922 at 1/200 dilution in immunohistochemical analysis, followed by a fluorescent secondary antibody.
Paraffin-embedded human breast cancer tissue stained for Peroxiredoxin 2/PRP using ab226922 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226922 has not yet been referenced specifically in any publications.