Product nameAnti-Peroxiredoxin 2/PRP antibody
See all Peroxiredoxin 2/PRP primary antibodies
DescriptionRabbit polyclonal to Peroxiredoxin 2/PRP
Tested applicationsSuitable for: WB, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Cow, Pig, Rhesus monkey
Recombinant fragment within Human Peroxiredoxin 2/PRP (internal sequence). The exact sequence is proprietary.
Database link: P32119
- WB: A549 and HCT 116 whole cell extracts. IHC-P: Human breast cancer tissue. IHC-Fr: Rat liver tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 16,22 kDa.|
|IHC-Fr||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
FunctionInvolved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
Sequence similaritiesBelongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
- Information by UniProt
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All lanes : Anti-Peroxiredoxin 2/PRP antibody (ab226922) at 1/5000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 16,22 kDa
15% SDS-PAGE gel.
Frozen section of rat liver tissue stained for Peroxiredoxin 2/PRP using ab226922 at 1/200 dilution in immunohistochemical analysis, followed by a fluorescent secondary antibody.
Paraffin-embedded human breast cancer tissue stained for Peroxiredoxin 2/PRP using ab226922 at 1/500 dilution in immunohistochemical analysis.
ab226922 has not yet been referenced specifically in any publications.