Recombinant HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197042)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR5154] to Peroxiredoxin 2/PRP
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154]
See all Peroxiredoxin 2/PRP primary antibodies -
Description
HRP Rabbit monoclonal [EPR5154] to Peroxiredoxin 2/PRP -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293, HeLa, LNCaP and SH-SY5Y whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5154 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/3000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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Notes |
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WB
1/3000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Function
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). -
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7001 Human
- Entrez Gene: 21672 Mouse
- Entrez Gene: 29338 Rat
- Omim: 600538 Human
- SwissProt: P32119 Human
- SwissProt: Q61171 Mouse
- SwissProt: P35704 Rat
- Unigene: 432121 Human
see all -
Alternative names
- Epididymis secretory sperm binding protein Li 2a antibody
- HEL S 2a antibody
- MGC4104 antibody
see all
Images
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All lanes : HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197042) at 1/3000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PRDX2 (Peroxiredoxin 2) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 22 kDa
Exposure time: 20 minutesab197042 was shown to specifically react with PRDX2 in wild-type HAP1 cells as signal was lost in PRDX2 (Peroxiredoxin 2) knockout cells. Wild-type and PRDX2 (Peroxiredoxin 2) knockout samples were subjected to SDS-PAGE. Ab197042 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/3000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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All lanes : HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197042) at 1/3000 dilution
Lane 1 : HEK-293 (Human) Whole Cell Lysate (ab52256)
Lane 2 :HeLa whole cell lysate (ab150035)
Lane 3 : LNCaP (Human prostate adenocarcinoma cell line) Whole Cell Lysate - clone FGC
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197042 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab197042 has not yet been referenced specifically in any publications.