Overview

  • Product name

    Anti-Peroxiredoxin 2/PRP antibody [EPR5155]
    See all Peroxiredoxin 2/PRP primary antibodies
  • Description

    Rabbit monoclonal [EPR5155] to Peroxiredoxin 2/PRP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Peroxiredoxin 2/PRP aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P32119

  • Positive control

    • 293T, HepG2, LnCaP and U937 cell lysates; HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
    • Sequence similarities

      Belongs to the ahpC/TSA family.
      Contains 1 thioredoxin domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Epididymis secretory sperm binding protein Li 2a antibody
      • HEL S 2a antibody
      • MGC4104 antibody
      • Natural killer cell enhancing factor B antibody
      • Natural killer cell-enhancing factor B antibody
      • Natural Killer Enhancing Factor B antibody
      • NKEF B antibody
      • NKEF-B antibody
      • NKEFB antibody
      • Peroxiredoxin 2 antibody
      • Peroxiredoxin-2 antibody
      • PRDX 2 antibody
      • PRDX2 antibody
      • PRDX2_HUMAN antibody
      • PrP antibody
      • PRX2 antibody
      • PRXII antibody
      • PTX1 antibody
      • TDPX1 antibody
      • Thiol Specific Antioxidant 1 antibody
      • Thiol specific antioxidant protein antibody
      • Thiol-specific antioxidant protein antibody
      • Thioredoxin Dependent Peroxide Reductase 1 antibody
      • Thioredoxin peroxidase 1 antibody
      • Thioredoxin-dependent peroxide reductase 1 antibody
      • Torin antibody
      • TPX1 antibody
      • TSA antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Peroxiredoxin 2/PRP knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: LnCaP cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab133481 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab133481 was shown to specifically react with Peroxiredoxin 2/PRP when Peroxiredoxin 2/PRP knockout samples were used. Wild-type and Peroxiredoxin 2/PRP knockout samples were subjected to SDS-PAGE. ab133481 and ab8245 (loading control to GAPDH) were diluted 1/50 000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
      and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Overlay histogram showing HeLa cells stained with ab133481 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133481, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481) at 1/50000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : LnCaP cell lysate
      Lane 4 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 22 kDa

    • Immunofluorescence analysis of HeLa cells labelling Peroxiredoxin 2/PRP with ab133481 at 1/100 dilution.

    References

    This product has been referenced in:

    • Bera R  et al. Genetic and Epigenetic Perturbations by DNMT3A-R882 Mutants Impaired Apoptosis through Augmentation of PRDX2 in Myeloid Leukemia Cells. Neoplasia 20:1106-1120 (2018). Read more (PubMed: 30245403) »
    • Duan T  et al. Role of peroxiredoxin 2 in H2O2-induced oxidative stress of primary Leydig cells. Mol Med Rep 13:4807-13 (2016). Read more (PubMed: 27082744) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (red blood cell)
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Loading amount
    20 µg
    Specification
    red blood cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Takujiro Homma

    Verified customer

    Submitted Dec 10 2018

    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - whole cell (2- 32 cell stage embryo)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    15 µg
    Treatment
    NEM to block reduced thiols.
    Specification
    2- 32 cell stage embryo
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 17°C

    Abcam user community

    Verified customer

    Submitted Sep 21 2018

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    No
    Fixative
    Methanol

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 10 2013

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