Anti-Peroxiredoxin 3/PRDX3 antibody (ab73349)
Key features and details
- Rabbit polyclonal to Peroxiredoxin 3/PRDX3
- Suitable for: IHC-P, ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Peroxiredoxin 3/PRDX3 antibody
See all Peroxiredoxin 3/PRDX3 primary antibodies -
Description
Rabbit polyclonal to Peroxiredoxin 3/PRDX3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Chimpanzee, Zebrafish, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Peroxiredoxin 3/PRDX3 aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab85815) -
Positive control
- Recombinant human Peroxiredoxin 3/PRDX3 protein (ab85559) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Human, Mouse and Rat Liver, Rat Ovary.
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General notes
This product was previously labelled as Peroxiredoxin 3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab73349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 28 kDa). |
Target
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Function
Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. -
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 281998 Cow
- Entrez Gene: 10935 Human
- Entrez Gene: 11757 Mouse
- Entrez Gene: 100174238 Orangutan
- Entrez Gene: 64371 Rat
- Omim: 604769 Human
- SwissProt: P35705 Cow
- SwissProt: P30048 Human
see all -
Alternative names
- Antioxidant protein 1 antibody
- AOP 1 antibody
- AOP-1 antibody
see all
Images
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Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody (ab73349)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Peroxiredoxin 3/PRDX3 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab73349 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.ab73349 was shown to specifically react with Peroxiredoxin 3/PRDX3 in wild-type HAP1 cells. No band was observed when Peroxiredoxin 3/PRDX3 knockout samples were examined. Wild-type and Peroxiredoxin 3/PRDX3 knockout samples were subjected to SDS-PAGE. ab73349 and ab8245 (loading control to GAPDH) were diluted at 1μg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody (ab73349)All lanes : Anti-Peroxiredoxin 3/PRDX3 antibody (ab73349) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : Ovary (Rat) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 52 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 3/PRDX3 antibody (ab73349)ICC/IF image of ab73349 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73349, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody (ab73349)IHC image of Peroxiredoxin 3/PRDX3 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73349, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Protocols
References (23)
ab73349 has been referenced in 23 publications.
- Gao J et al. Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase a2. Redox Biol 28:101345 (2020). PubMed: 31669973
- Stein KT et al. Kinetic modeling of H2O2 dynamics in the mitochondria of HeLa cells. PLoS Comput Biol 16:e1008202 (2020). PubMed: 32925922
- Torrente L et al. Inhibition of TXNRD or SOD1 overcomes NRF2-mediated resistance to ß-lapachone. Redox Biol 30:101440 (2020). PubMed: 32007910
- Trstenjak Prebanda M et al. Upregulation of Mitochondrial Redox Sensitive Proteins in LPS-Treated Stefin B-Deficient Macrophages. Cells 8:N/A (2019). PubMed: 31766320
- Booty LM et al. Selective Disruption of Mitochondrial Thiol Redox State in Cells and In Vivo. Cell Chem Biol 26:449-461.e8 (2019). PubMed: 30713096
