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Hello, I ordered Peroxiredoxin 3 antibody ab 16751 a few months ago. When I did western blot I detected a band at 22 KDa and few non-specific between 50-60 Kda. According to me it seems like I was sent ab73349, which is the other peroxiredoxin 3 antibody sold by abcam. My worry is that since ab73349 detects 22 Kda band, is it really detecting Peroxiredoxin 3? What's known about this antibody production. I'm in my finishing stages of PhD and peroxiredoxin 3 is the last western I need to do. Could you please help me with this antibody. Thanks,
Asked on Nov 07 2011
Thank you for contacting us. I am sorry to hearing about the confusion. We never send wrong products to our customers so if you have ordered antibody ab16751 then the antibody in the vial will be Anti-Peroxiredoxin 3 antibody [12B] (ab16751). We do send different products in cases where the products are out of stock but not without confirmation from our customers. We always contact them by phone or by email for their choice of waiting or selecting another product from catalogue. The ab16751 was in stock when you ordered it so there is not room for error from our end. I also have few questions which will help me to understand the problem: Ab16751 is a Mouse monoclonal and ab73349 is a Rabbit polyclonal. Could you specify which secondary antibody you have used; was it anti mouse or anti rabbit. The extra bands between 50-60 kDa do not mean that the antibody is different in the vial. These bands could be due to different reasons e.g. high conc. of primary or secondary antibody, loading high conc. of lysates, not using protease inhibitors in lysis buffer etc. We have observed 2 extra bands in case of ab73349 with Rat liver lysates; are you using the same lysates? Finally I would suggest troubleshooting the experiment. I can also help you with troubleshooting however I would need more information about the protocol used. Please fill in the attached questionnaire; I will then check the protocol for possible optimizations. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Nov 07 2011