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Thank you for your reply. I did use mouse secondary antibody and thank you for pointing out that the other antibody is rabbit polyclonal. Would you have any idea at what molecular weight does mouse peroxiredoxin 3 migrate on a gel? The signal is very real on my western blot the only worry is that it's not on the predicted molecular weight. Since ab73349 picks up a band at 22 Kda it made me wonder perhaps the mouse peroxiredoxin migrates at a 22 KDa. Thanks,
Asked on Nov 09 2011
Thank you for contacting us. The antibody should be detecting a band around 27 kDa. I would suggest searching and literature and comparing with other people findings. The NCBI Ref seq actually gives info of two isoforms which are slightly different to each other. It may be possible that the antibody is detecting a different isoform than canonical. It is just a thought; you may need to check the literature or running further tests would also be an option. I am sorry I can’t be more specific as I don’t have information of protocol you have been using. Please check the following links: http://www.ncbi.nlm.nih.gov/protein/NP_006784.1 http://www.uniprot.org/uniprot/P30048 http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111109-155629-0237-39221683-pg I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Nov 09 2011