Overview

  • Product name

    Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114]
    See all Peroxiredoxin 3/PRDX3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8114] to Peroxiredoxin 3/PRDX3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Peroxiredoxin 3/PRDX3 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, MCF-7, and A431 cell lysates; Human liver and thyroid gland carcinoma tissues; MCF-7 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Peroxiredoxin 3

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P 1/50 - 1/100.
WB 1/50000 - 1/200000. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
    • Sequence similarities

      Belongs to the ahpC/TSA family.
      Contains 1 thioredoxin domain.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • Antioxidant protein 1 antibody
      • AOP 1 antibody
      • AOP-1 antibody
      • AOP1 antibody
      • HBC189 antibody
      • MER5 antibody
      • MGC104387 antibody
      • MGC24293 antibody
      • mitochondrial antibody
      • peroxiredoxin 3 antibody
      • Peroxiredoxin III antibody
      • Peroxiredoxin-3 antibody
      • PRDX3 antibody
      • PRDX3_HUMAN antibody
      • PRO1748 antibody
      • Protein MER5 homolog antibody
      • PRX III antibody
      • Prx-III antibody
      • PRX3 antibody
      • SP 22 antibody
      • SP-22 antibody
      • SP22 antibody
      • Thioredoxin dependent peroxide reductase mitochondrial antibody
      • Thioredoxin-dependent peroxide reductase antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Peroxiredoxin 3/PRDX3 knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF7 cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab129206 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab129206 was shown to specifically react with Peroxiredoxin 3/PRDX3 when Peroxiredoxin 3/PRDX3 knockout samples were used. Wild-type and Peroxiredoxin 3/PRDX3 knockout samples were subjected to SDS-PAGE. ab129206 and ab8245 (loading control to GAPDH) were both diluted 1/50000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in MCF-7 cells by Immunofluorescence.

    • Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling Peroxiredoxin 3/PRDX3 with unpurified ab129206 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • All lanes : Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 26 kDa
      why is the actual band size different from the predicted?

    • ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin-embedded Human liver tissue by Immunohistochemistry.

    • ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.

    References

    ab129206 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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