Anti-Peroxiredoxin 4 antibody (ab15574)
Key features and details
- Rabbit polyclonal to Peroxiredoxin 4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Peroxiredoxin 4 antibody
See all Peroxiredoxin 4 primary antibodies -
Description
Rabbit polyclonal to Peroxiredoxin 4 -
Host species
Rabbit -
Specificity
This antibody is specific for Peroxiredoxin 4 and shows no cross reactivity with other Peroxiredoxin isoforms. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Peroxiredoxin 4. Purified human recombinant Peroxiredoxin 4 from K562 cells.
Database link: Q13162 -
Positive control
- WB: MCF7, DU-145, PC-3, LNCaP, MDA-MB-231, HepG2, A549 and U-937 membrane enriched extract lysate. ICC/IF: LNCaP cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 31 kDa).
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| ICC/IF | (2) |
1/250.
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| Notes |
|---|
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WB
1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 31 kDa). |
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ICC/IF
1/250. |
Target
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Function
Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. -
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10549 Human
- Omim: 606506 Human
- SwissProt: Q13162 Human
- Unigene: 83383 Human
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Alternative names
- Antioxidant enzyme 372 antibody
- Antioxidant enzyme AOE372 antibody
- AOE37 2 antibody
see all
Images
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Western blot - Anti-Peroxiredoxin 4 antibody (ab15574)All lanes : Anti-Peroxiredoxin 4 antibody (ab15574) at 1/5000 dilution
Lane 1 : MCF7 membrane enriched extract lysate
Lane 2 : DU-145 membrane enriched extract lysate
Lane 3 : PC-3 membrane enriched extract lysate
Lane 4 : LNCaP membrane enriched extract lysate
Lane 5 : MDA-MB-231 membrane enriched extract lysate
Lane 6 : HepG2 membrane enriched extract lysate
Lane 7 : A549 membrane enriched extract lysate
Lane 8 : U-937 membrane enriched extract lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 31 kDaThe blot was probed with primary antibody (ab15574) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (0.4 µg/mL, 1/2500 dilution).
This antibody detects an ~23 kDa and ~20 kDa protein representing pro-Peroxiredoxin 4 and mature Peroxiredoxin 4, respectively, in human PC-3 lysate.
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Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 4 antibody (ab15574)Immunofluorescence analysis of Peroxiredoxin 4 was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRDX4 Rabbit Polyclonal Antibody (ab15574) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (1/300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab15574 has been referenced in 4 publications.
- Sadvakassova G et al. Active hematopoiesis triggers exosomal release of PRDX2 that promotes osteoclast formation. Physiol Rep 9:e14745 (2021). PubMed: 33587325
- Lombardi R et al. HSP90 identified by a proteomic approach as druggable target to reverse platinum resistance in ovarian cancer. Mol Oncol 15:1005-1023 (2021). PubMed: 33331136
- Kuljittichanok D et al. Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncol Lett 16:1943-1952 (2018). PubMed: 30034552
- Palande K et al. Peroxiredoxin-controlled G-CSF signalling at the endoplasmic reticulum-early endosome interface. J Cell Sci 124:3695-705 (2011). PubMed: 22045733
