Anti-Peroxiredoxin 4 antibody (ab15574)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Peroxiredoxin 4 antibody
See all Peroxiredoxin 4 primary antibodies -
DescriptionRabbit polyclonal to Peroxiredoxin 4
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Host speciesRabbit
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SpecificityThis antibody is specific for Peroxiredoxin 4 and shows no cross reactivity with other Peroxiredoxin isoforms.
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Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Other Immunogen Type corresponding to Human Peroxiredoxin 4. Purified human recombinant Peroxiredoxin 4 from K562 cells.
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Positive control
- PC3 cell lysate (WB) TSU-Pr1 cells (ICC)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
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PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab15574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 33 kDa). Detects a band of approximately 23 kDa and 20 kDa representing Pro-Peroxiredoxin 4 and mature Peroxiredoxin 4 respectively. |
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| ICC | 1/500. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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FunctionProbably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
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Sequence similaritiesBelongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Cellular localizationCytoplasm. Secreted.
- Information by UniProt
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Database links
- Entrez Gene: 10549 Human
- Omim: 606506 Human
- SwissProt: Q13162 Human
- Unigene: 83383 Human
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Alternative names
- Antioxidant enzyme 372 antibody
- Antioxidant enzyme AOE372 antibody
- AOE37 2 antibody
see all
Images
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Immunocytochemistry - Anti-Peroxiredoxin 4 antibody (ab15574)ab15574 staining Peroxiredoxin 4 in TSU-Pr1 cells. -
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 4 antibody (ab15574)This image is courtesy of an anonymous Abreviewab15574 staining Peroxiredoxin 4 (green) in Mouse bone marrow by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and incubated with primary antibody (1/100 in 0.05% Saponin/PBS) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit IgG polyclonal (1/10000) was used as the secondary antibody.
Datasheets and documents
References
This product has been referenced in:
- Kuljittichanok D et al. Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncol Lett 16:1943-1952 (2018). Read more (PubMed: 30034552) »
- Palande K et al. Peroxiredoxin-controlled G-CSF signalling at the endoplasmic reticulum-early endosome interface. J Cell Sci 124:3695-705 (2011). Read more (PubMed: 22045733) »
